|
| Status |
Public on Dec 31, 2013 |
| Title |
β-catenin target genes in colorectal carcinoma cell lines with deregulated Wnt/β-catenin signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To validate the suitability of two commonly used colorectal cancer cell lines, DLD1 and SW480, as model systems to study colorectal carcinogenesis, we treated these cell lines with β-catenin siRNA and identified β-catenin target genes using DNA microarrays. The list of identified target genes was compared to previously published β-catenin target genes found in the PubMed and the GEO databases.
Based on the large number of β-catenin target genes found to be similarly regulated in DLD1, SW480 and LS174T as well as the large overlap with confirmed β-catenin target genes, we conclude that DLD1 and SW480 colon carcinoma cell lines are suitable model systems to study β-catenin regulated genes and signaling pathways
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| |
|
| Overall design |
12 arrays (2 cell lines, 2 treatments, 3 biological replicates)
|
| |
|
| Contributor(s) |
Herbst A, Jurinovic V, Krebs S, Thieme SE, Blum H, Kolligs FT |
| Citation(s) |
24467841 |
| |
| Submission date |
Feb 05, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Stefan Krebs |
| E-mail(s) |
[email protected]
|
| Phone |
0049-89-2180 76715
|
| Organization name |
Ludwig-Maximilian University
|
| Department |
Gene Center
|
| Lab |
Lafuga Genomics
|
| Street address |
Feodor-Lynen-Str. 25
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA188581 |
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