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| Status |
Public on Jan 16, 2014 |
| Title |
The Genomics of the Fetal Hypothalamic Cellular Response to Transient Hypoxia: Endocrine, Immune, and Metabolic Responses |
| Organism |
Ovis aries |
| Experiment type |
Expression profiling by array
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| Summary |
Fetuses respond to transient hypoxia (a common stressor in utero) with cellular responses that are appropriate for promoting survival of the fetus. The present experiment was performed to identify the acute genomic responses of the fetal hypothalamus to transient hypoxia. Three fetal sheep were exposed to 30 min of hypoxia and hypothalamic mRNA extracted from samples collected 30 min after return to normoxia. These samples were compared to those from 4 normoxic control fetuses using the Agilent 019921 ovine array. Differentially-regulated genes were analyzed by network analysis and by gene ontology analysis, identifying statistically significant overrepresentation of biological processes. Real-time PCR of selected genes supported the validity of the array data. Hypoxia induced increased expression of genes involved in response to oxygen stimulus, RNA splicing, anti-apoptosis, vascular smooth muscle proliferation, and positive regulation of Notch receptor target. Downregulated genes were involved in metabolism, antigen receptor-mediated immunity, macromolecular complex assembly, S-phase, translation elongation, RNA splicing, protein transport, and post-transcriptional regulation. We conclude that these results emphasize that the cellular response to hypoxia involves reduced metabolism, the involvement of the fetal immune system, and the importance of glucocorticoid signaling.
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| Overall design |
3 Ventilatory Hypoxia (VH) and 4 Control (con) fetuses. All fetuses were chronically catheterized and in late gestation. Hypoxia produced by low PO2 in maternal inspired gas for 30 min, followed by normoxia recovery for 30 min. Control fetuses maintained at normoxia for 30 min, followed by another 30 min of normoxia. Hypothalami collected for mRNA at end of normoxic recovery period.
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| Contributor(s) |
Wood CE, Rabaglino MB, Chang E, Denslow N, Keller-Wood M, Richards E |
| Citation(s) |
23653468 |
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| Submission date |
Jan 09, 2013 |
| Last update date |
Jan 16, 2014 |
| Contact name |
Charles Evans Wood |
| E-mail(s) |
[email protected]
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| Phone |
352-294-5064
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| Organization name |
University of Florida
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| Department |
Physiology and Functional Genomics
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| Street address |
1345 Center Drive/Room M552
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| City |
GAINESVILLE |
| State/province |
Florida |
| ZIP/Postal code |
32610-0274 |
| Country |
USA |
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| Platforms (1) |
| GPL10427 |
Agilent-019921 Sheep Gene Expression Microarray (Feature Number version) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA185757 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43371_RAW.tar |
19.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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