|
| Status |
Public on May 29, 2013 |
| Title |
Cell- and tissue-specific transcriptome analyses of Medicago truncatula root nodules |
| Platform organisms |
Sinorhizobium meliloti; Medicago sativa; Medicago truncatula |
| Sample organism |
Medicago truncatula |
| Experiment type |
Expression profiling by array
|
| Summary |
We used laser-capture microdissection (LCM) to isolate specific cells from the Medicago truncatula nodule meristem (M), the distal infection (DIZ), the proximal infection zone (PIZ), infected cells (IC) and uninfected cells (UIC) from the fixation zone.
Based on Medicago GeneChips, we identified the cell- and tissue-specific programm of gene expression in Medicago truncatula root nodules.
|
| |
|
| Overall design |
Nodules were harvested three weeks after inoculation of Medicago truncatula (genotype Jemalong A17) plants with Sinorhizobium meliloti strain 2011. Nodules were fixed in Farmer's fixative and subsequently embedded in paraffin. 8 µm de-paraffined sections were used to capture cells from the nodule meristem, distal infection zone, proximal infection zone, infected cells and uninfected cells from the fixation zone, using an Arcturus Pixcell II laser capture microscope. 3 biological replicates were used for each cell-/tissue type. After RNA extraction, the RNA was amplified and used for Medicago Gene Chip hybridization.
|
| |
|
| Contributor(s) |
Limpens E, Moling S, Hooiveld G, Pereira PA, Bisseling T, Becker JD, Küster H |
| Citation(s) |
23734198 |
| |
| Submission date |
Jan 08, 2013 |
| Last update date |
Jun 05, 2013 |
| Contact name |
Helge Küster |
| E-mail(s) |
[email protected]
|
| Organization name |
Leibniz Universität Hannover
|
| Department |
Institut für Pflanzengenetik
|
| Lab |
Abt. IV - Pflanzengenomforschung
|
| Street address |
Herrenhäuser Str. 2
|
| City |
Hannover |
| ZIP/Postal code |
30419 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL4652 |
[Medicago] Affymetrix Medicago Genome Array |
|
| Samples (15)
|
| GSM1060861 |
Meristem cells from Medicago truncatula root nodules replicate 4 |
| GSM1060862 |
Meristem cells from Medicago truncatula root nodules replicate 1 |
| GSM1060863 |
Meristem cells from Medicago truncatula root nodules replicate 3 |
| GSM1060864 |
Distal infection zone cells from Medicago truncatula root nodules replicate 1 |
| GSM1060865 |
Distal infection zone cells from Medicago truncatula root nodules replicate 2 |
| GSM1060866 |
Distal infection zone cells from Medicago truncatula root nodules replicate 3 |
| GSM1060867 |
Proximal infection zone cells from Medicago truncatula root nodules replicate 1 |
| GSM1060868 |
Proximal infection zone cells from Medicago truncatula root nodules replicate 2 |
| GSM1060869 |
Proximal infection zone cells from Medicago truncatula root nodules replicate 3 |
| GSM1060870 |
Infected cells from Medicago truncatula root nodules replicate 1 |
| GSM1060871 |
Infected cells from Medicago truncatula root nodules replicate 3 |
| GSM1060872 |
Infected cells from Medicago truncatula root nodules replicate 2 |
| GSM1060873 |
Uninfected cells from Medicago truncatula root nodules replicate 1 |
| GSM1060874 |
Uninfected cells from Medicago truncatula root nodules replicate 2 |
| GSM1060875 |
Uninfected cells from Medicago truncatula root nodules replicate 3 |
|
| Relations |
| BioProject |
PRJNA185501 |
Less...
More...