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| Status |
Public on Feb 20, 2013 |
| Title |
Cellobiose-mediated gene expression in Streptococcus pneumoniae and the repressor function of the novel GntR-type regulator BguR |
| Organism |
Streptococcus pneumoniae D39 |
| Experiment type |
Expression profiling by array
|
| Summary |
The human pathogen Streptococcus pneumoniae has the ability to use the carbon- and energy source cellobiose due to the presence of a cellobiose-utilizing gene cluster (cel locus) in its genome. This system is regulated by the cellobiose-dependent transcriptional activator CelR, which has been previously shown to contribute to pneumococcal virulence. To get a broader understanding of the response of S. pneumoniae to cellobiose, we compared the pneumococcal transcriptome during growth on glucose as the main carbon source to that with cellobiose as the main carbon source. The expression of various carbon metabolic genes was altered, including a PTS operon (which we here denote as the bgu operon) that has high similarity with the cel locus. In contrast to the cel locus, the bgu operon is conserved in all sequenced strains of S. pneumoniae, indicating an important physiological function in the lifestyle of pneumococci. We next characterized the transcriptional regulation of the bgu operon in more detail. Its expression was increased in the presence of cellobiose, and decreased in the presence of glucose. A novel GntR-type transcriptional regulator (which we here denote as BguR) was shown to act as a transcriptional repressor of the bgu operon and its repressive effect was relieved in the presence of cellobiose. BguR-dependent repression was demonstrated to be mediated by a 20-bp DNA operator site (5’-AAAAATGTCTAGACAAATTT-3’) present in PbguA as verified by promoter truncation experiments. In conclusion, we have identified a new cellobiose-responsive PTS operon, together with its transcriptional regulator in S. pneumoniae.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series
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| |
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| Citation(s) |
23469031 |
| |
| Submission date |
Jan 08, 2013 |
| Last update date |
Nov 02, 2016 |
| Contact name |
Sulman Shafeeq |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institutet
|
| Department |
MTC
|
| Street address |
nobel vag 16
|
| City |
STOCKHOLM |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL11484 |
Streptococcus pneumoniae D39 Multiplatform 1909k array |
|
| Samples (9)
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| This SuperSeries is composed of the following SubSeries: |
| GSE43342 |
Impact of bguR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose |
| GSE43343 |
Transcriptional response of Streptococcus pneumoniae D39 to Cellobiose at time point T1 |
| GSE43344 |
Transcriptional response of Streptococcus pneumoniae D39 to Cellobiose at time point T2 |
|
| Relations |
| BioProject |
PRJNA185486 |
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