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| Status |
Public on Dec 25, 2012 |
| Title |
Real-time quantitative PCR analysis of mRNA from lungs of WT and Pglyrp1-/- mice sensitized with HDM |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Wild type (WT) and Pglyrp1-/- mice were treated with PBS or sensitized 5 days/week for 3 or 5 weeks with 10 µl per application of 2.5 mg/ml of purified house dust mite allergen. 3 days after the last sensitization the lungs were removed and homogenized, and RNA was isolated from the right lobes using the TRIZOL method. Quantitative reverse transcription real-time PCR (qRT-PCR) was used to quantify the amounts of mRNA in the lungs using custom RT2 Profiler PCR Arrays designed by us and manufactured by Qiagen/SA Biosciences.
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| Overall design |
qRT-PCR gene expression profiling
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| Contributor(s) |
Park SY, Gupta D, Dziarski R |
| Citation(s) |
23420883 |
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| Submission date |
Dec 24, 2012 |
| Last update date |
Mar 07, 2013 |
| Contact name |
Roman Dziarski |
| E-mail(s) |
[email protected]
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| Phone |
219-980-6535
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| Organization name |
Indiana University School of Medicine-Northwest
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| Street address |
3400 Broadway
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| City |
Gary |
| State/province |
IN |
| ZIP/Postal code |
46408 |
| Country |
USA |
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| Platforms (1) |
| GPL16426 |
SA Biosciences Mouse lung gene expression array |
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| Samples (18)
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| GSM1057254 |
Pglyrp1-/- mice treated with PBS, expt 1 |
| GSM1057255 |
Pglyrp1-/- mice treated with PBS, expt 2 |
| GSM1057256 |
Pglyrp1-/- mice treated with PBS, expt 3 |
| GSM1057257 |
WT mice sensitized with HDM for 3 weeks, expt 1 |
| GSM1057258 |
WT mice sensitized with HDM for 3 weeks, expt 2 |
| GSM1057259 |
WT mice sensitized with HDM for 3 weeks, expt 3 |
| GSM1057260 |
Pglyrp1-/- mice sensitized with HDM for 3 weeks, expt 1 |
| GSM1057261 |
Pglyrp1-/- mice sensitized with HDM for 3 weeks, expt 2 |
| GSM1057262 |
Pglyrp1-/- mice sensitized with HDM for 3 weeks, expt 3 |
| GSM1057263 |
WT mice sensitized with HDM for 5 weeks, expt 1 |
| GSM1057264 |
WT mice sensitized with HDM for 5 weeks, expt 2 |
| GSM1057265 |
WT mice sensitized with HDM for 5 weeks, expt 3 |
| GSM1057266 |
Pglyrp1-/- mice sensitized with HDM for 5 weeks, expt 1 |
| GSM1057267 |
Pglyrp1-/- mice sensitized with HDM for 5 weeks, expt 2 |
| GSM1057268 |
Pglyrp1-/- mice sensitized with HDM for 5 weeks, expt 3 |
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| Relations |
| BioProject |
PRJNA184701 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43137_fold_change.txt.gz |
2.0 Kb |
(ftp)(http) |
TXT |
| GSE43137_non_normalized.txt.gz |
2.8 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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