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| Status |
Public on Apr 28, 2013 |
| Title |
A roadmap of cell-type specific gene expression during early stages of the arbuscular mycorrhiza symbiosis |
| Platform organisms |
Sinorhizobium meliloti; Medicago sativa; Medicago truncatula |
| Sample organism |
Medicago truncatula |
| Experiment type |
Expression profiling by array
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| Summary |
Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel cell-type specific gene expression during early stages of Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on laser-microdissected cells. We used Medicago GeneChips to detail the cell-type specific programme of gene expression in early stages of colonization by arbuscular mycorrhizal fungi and identified genes differentially expressed during these stages.
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| Overall design |
Medicago truncatula Gaertn ‘Jemalong’ genotype A17 plantlets were grown in the climate chamber. Plants grown for the collection appressorial root areas (APP) and the corresponding non-appressorial root areas (NAP) were mycorrhized after 3 weeks and roots were harvested at 5-6 dpi.
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| Contributor(s) |
Hogekamp C, Becker JD, Küster H |
| Citation(s) |
23647797 |
| |
| Submission date |
Dec 05, 2012 |
| Last update date |
May 08, 2013 |
| Contact name |
Helge Küster |
| E-mail(s) |
[email protected]
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| Organization name |
Leibniz Universität Hannover
|
| Department |
Institut für Pflanzengenetik
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| Lab |
Abt. IV - Pflanzengenomforschung
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| Street address |
Herrenhäuser Str. 2
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| City |
Hannover |
| ZIP/Postal code |
30419 |
| Country |
Germany |
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| Platforms (1) |
| GPL4652 |
[Medicago] Affymetrix Medicago Genome Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE42748 |
A roadmap of cell-type specific gene expression during sequential stages of the arbuscular mycorrhiza symbiosis |
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| Relations |
| BioProject |
PRJNA182993 |
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