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| Status |
Public on Apr 27, 2013 |
| Title |
Expression profiling of Ciona intestinalis neurula and mid-tailbud stage embryos expressing altered forms of the transcription factor Ci-Tbx2/3 in the notochord |
| Organism |
Ciona intestinalis |
| Experiment type |
Expression profiling by array
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| Summary |
Despite being found in the notochord of several chordates, the roles of the Tbx2 subfamily of T-box transcription factors in the development of this tissue remain largely unknown. We explored the targets of the only Tbx2 subfamily member in Ciona intestinalis, Ci-Tbx2/3, by expressing mutant forms of the transcriptional regulator using the Ci-Bra promoter region.
We produced a dominant interfering version of Ci-Tbx2/3 (Tbx2/3DBD) through expression of a truncated version consisting only of its DNA-binding domain (DBD) and a constitutive activator form by attaching the T-box of Ci-Tbx2/3 to the VP16 transactivation domain (Tbx2/3VP16). These constructs were introduced into 1-cell stage embryos and grown to the neurula (N) or mid-tailbud (mTb) stage to capture targets regulated throughout notochord morphogenesis.
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| Overall design |
Ci-Tbx2/3 targets were ascertained using whole-genome custom Affymetrix microarrays to compare the transcription levels of Tbx2/3DBD and Tbx2/3VP16 expressing embryos to wild-type controls (Bra>GFP) at the neurula or mTb stages.
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| Contributor(s) |
Di Gregorio A |
| Citation(s) |
23674602 |
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| Submission date |
Nov 14, 2012 |
| Last update date |
Jul 03, 2013 |
| Contact name |
Anna Di Gregorio |
| E-mail(s) |
[email protected]
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| Phone |
212-998-9564
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| Organization name |
NYU College of Dentistry
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| Department |
Basic Science and Craniofacial Biology
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| Lab |
Di Gregorio
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| Street address |
345 E 24th Street, room 804A
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10010 |
| Country |
USA |
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| Platforms (1) |
| GPL15657 |
[CINT06a520380F] Affymetrix Ciona intestinalis custom-designed array |
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| Samples (17)
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| GSM1036466 |
Tbx2/3 DBD at N, replicate 1 |
| GSM1036467 |
Tbx2/3 DBD at N, replicate 2 |
| GSM1036468 |
Tbx2/3 DBD at N, replicate 3 |
| GSM1036469 |
Tbx2/3 VP16 at N, replicate 1 |
| GSM1036470 |
Tbx2/3 VP16 at N, replicate 2 |
| GSM1036471 |
Tbx2/3 VP16 at N, replicate 3 |
| GSM1036472 |
control at mTb, replicate 1 |
| GSM1036473 |
control at mTb, replicate 2 |
| GSM1036474 |
Tbx2/3 DBD at mTb, replicate 1 |
| GSM1036475 |
Tbx2/3 DBD at mTb, replicate 2 |
| GSM1036476 |
Tbx2/3 DBD at mTb, replicate 3 |
| GSM1036477 |
Tbx2/3 VP16 at mTb, replicate 1 |
| GSM1036478 |
Tbx2/3 VP16 at mTb, replicate 2 |
| GSM1036479 |
Tbx2/3 VP16 at mTb, replicate 3 |
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| Relations |
| BioProject |
PRJNA181103 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42267_RAW.tar |
66.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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