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| Status |
Public on Nov 01, 2013 |
| Title |
Methylomic analysis identifies the involvement of migration and adhesion genes in the ageing of primary haematopoietic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
we utilize Nano-MeDIP-seq for the analysis of the LT-HSC methylome and, for the first time, simultaneously interrogate the methylome and transcriptome of a homogeneous population of primary murine HSCs, in order to define the underlying causes of changes in HSC functionality during normal ageing.
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| Overall design |
We isolated and phenotyped primary LT-HSCs from young, middle-aged and old mice and subjected them to comprehensive methylome (MeDIP-seq) and transcriptome (RNA-seq) analysis.
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| Contributor(s) |
Taiwo O, Wilson GA, Emmett W, Morris T, Bonnet D, Adejumo T, Pearce D, Beck S |
| Citation(s) |
23949429 |
| |
| Submission date |
Oct 17, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Gareth Wilson |
| E-mail(s) |
[email protected]
|
| Organization name |
UCL Cancer Institute
|
| Street address |
23 Huntley Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA177844 |
| SRA |
SRP016510 |
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