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| Status |
Public on May 01, 2006 |
| Title |
Basonuclin: A novel mammalian maternal effect gene |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Basonuclin, which is a zinc-finger protein found in abundance only in the keratinocytes of the stratified epithelium, male germ cells and oocytes, qualifies as a maternal-effect gene because the source of pre-implantation embryonic basonuclin is maternal. Using a transgenic-RNAi approach, we knocked-down basonuclin specifically in mouse oocytes, which led to female sub-fertility. Basonuclin deficiency in oocytes perturbed both RNA polymerase I- and II-mediated transcription and oocyte morphology was affected as evidenced by cytoplasmic and cell surface abnormalities. The affected oocytes, however, could still mature to and arrest at metaphase II and be ovulated, suggesting the impaired pathways were not essential for oocyte development and maturation. Nevertheless, an early embryonic failure in pre-implantation development was identified and likely accounted for the sub-fertility phenotype. These results suggest that basonuclin is a new member of the mammalian maternal-effect genes and interestingly, differs from the previously reported mammalian maternal-effect genes in that it also apparently perturbs oogenesis.
Keywords: RNAi knockdown
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| Overall design |
Basonuclin-deficient oocytes were created by a mouse transgenic RNAi approach, which knocks down basonuclin mRNA level specifically in oocytes. Microarray analysis was performed on total RNA from four non-transgenic (control) and six transgenic (basonuclin-deficient) oocyte samples (approximately 50 oocytes per sample).
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| Contributor(s) |
Ma J, Zeng F, Schultz RM, Tseng H |
| Citation(s) |
16624857 |
| |
| Submission date |
Jan 13, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Hung Tseng |
| E-mail(s) |
[email protected]
|
| Phone |
215 898 1981
|
| Fax |
215 573 2033
|
| Organization name |
University of Pennsylvania
|
| Department |
Dermatology
|
| Lab |
Hung Tseng
|
| Street address |
415 Curie Boulevard Room 242B
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA95135 |
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