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| Status |
Public on Feb 25, 2015 |
| Title |
Transcriptome profiling reveals a genomewide role of G-proteins |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Knock out mutants of GPA1 (gpa1-5), GCR1 (gcr1-5) as well as their double mutant (gpa1-5 gcr1-5) were used to obtain genomewide transcriptome profile to deduce genomewide effect of both the subunits alone or in combination.
Heterotrimeric G-protein signaling pathways have been implicated in a variety of biological processes in plants. While an impressive range of important plant responses are known to be mediated by G-protein signaling pathways, the genes that respond through these pathways are not yet known completely. More importantly, the role of G-proteins in mediating plant metabolic regulation, which is well established in animal systems, has not received adequate attention. The availability of loss-of-function G_alpha (GPA1) and G-protein coupled receptor (GCR1) mutant(s) as well as their double mutant in Arabidopsis provided an opportunity for a genome-wide microarray analysis in the current study for the first time.
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| Overall design |
transcriptome profile of mutants gpa1-5, gcr1-5 and double mutant gpa1-5 gcr1-5 were compared to that of the corresponding wild type, ws2. biological duplicates of each sample wa used and hybridized using single color array
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| Contributor(s) |
Chakraborty N, Raghuram N |
| Citation(s) |
25668726, 26346778, 30967583 |
| |
| Submission date |
Aug 20, 2012 |
| Last update date |
Apr 12, 2019 |
| Contact name |
Nandula Raghuram |
| E-mail(s) |
[email protected], [email protected], [email protected], [email protected]
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| Phone |
+91-11-25302308
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| Organization name |
Guru Gobind Singh Indraprastha University
|
| Department |
School of Biotechnology
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| Lab |
Molecular Biology and Functional Genomics Lab
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| Street address |
Dwarka, Sector -16C
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| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110075 |
| Country |
India |
| |
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| Platforms (1) |
| GPL15928 |
Arabidopsis thaliana Whole Genome 8x60k designed by Genotypic Technology Pvt. Ltd. (AMADID:037661 ) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA173400 |
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