|
| Status |
Public on Jan 05, 2006 |
| Title |
Contribution of unique genes by individual cell lines to Universal Mouse Reference RNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
RNA from 11 individual mouse cell lines were reverse-transcribed to cDNA, labeled with Cy5 and cohybridized with Cy3-labeled UMRR onto 7,500-spot mouse oligo microarrays (UNC). The data was analyzed using GeneTraffic software. 300-1000 spots out of 8,000 were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics on only one microarray was determined.
A reference experiment design type is where all samples are compared to a common reference.
Keywords: reference_design
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| |
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| Overall design |
Computed
|
| |
|
| Contributor(s) |
Perou C |
| Citation(s) |
15113400 |
| |
| Submission date |
Jan 04, 2006 |
| Last update date |
Mar 17, 2012 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
[email protected]
|
| Phone |
650-498-6012
|
| URL |
http://genome-www5.stanford.edu/
|
| Department |
Stanford University, School of Medicine
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (14)
|
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| This SubSeries is part of SuperSeries: |
| GSE4389 |
Universal Reference RNA as a standard for microarray experiments |
|
| Relations |
| BioProject |
PRJNA104879 |
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