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Status |
Public on Jun 21, 2013 |
Title |
Genome wide profiling of RNA polymerase III in Saccharomyces cerevisiae |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Genome wide mapping of RNA polymearase III binding sites in Saccharomyces cerevisiae under normal growth and nutrient starved condition using ChIP-seq. Chromatin Immuno-precipitation (ChIP) was performed for FLAG tagged version of pol III subunit RPC128 after crosslinking the log-phase cells with formaldehyde. MOCK and IP DNA was sequenced and coverage of pol III was calculated at each base of the genome.
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Overall design |
RPC128-FLAG ChIP-seq single end seqquencing on Illumina GAII. 2 replicates of IP samples and 1 MOCK sample. Done in under normal growth and nutrient deprivation (4 hours).
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Contributor(s) |
Bhargava P, Kumar Y |
Citation(s) |
23767421 |
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Submission date |
Jul 23, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Yatendra Kumar |
E-mail(s) |
[email protected], [email protected]
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Organization name |
MRC Human Genetics Unit, The University Of Edinburgh
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Department |
MRC HGU
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Lab |
Wendy Bickmore
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Street address |
Crewe Road south
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City |
Edinburgh |
ZIP/Postal code |
Eh42xu |
Country |
United Kingdom |
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Platforms (1) |
GPL9377 |
Illumina Genome Analyzer II (Saccharomyces cerevisiae) |
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Samples (6)
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GSM971757 |
RPC128-FLAG IP_Normal Growth_Replicate1 |
GSM971758 |
RPC128-FLAG IP_Normal Growth_Replicate2 |
GSM971759 |
RPC128-FLAG MOCK_Normal Growth |
GSM971760 |
RPC128-FLAG IP_Nutrient Starvation_Replicate1 |
GSM971761 |
RPC128-FLAG IP_Nutrient Starvation_Replicate2 |
GSM971762 |
RPC128-FLAG MOCK_Nutrient Starvation |
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Relations |
BioProject |
PRJNA171220 |
SRA |
SRP014553 |