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Series GSE39566 Query DataSets for GSE39566
Status Public on Jun 21, 2013
Title Genome wide profiling of RNA polymerase III in Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Genome wide mapping of RNA polymearase III binding sites in Saccharomyces cerevisiae under normal growth and nutrient starved condition using ChIP-seq. Chromatin Immuno-precipitation (ChIP) was performed for FLAG tagged version of pol III subunit RPC128 after crosslinking the log-phase cells with formaldehyde. MOCK and IP DNA was sequenced and coverage of pol III was calculated at each base of the genome.
 
Overall design RPC128-FLAG ChIP-seq single end seqquencing on Illumina GAII. 2 replicates of IP samples and 1 MOCK sample. Done in under normal growth and nutrient deprivation (4 hours).
 
Contributor(s) Bhargava P, Kumar Y
Citation(s) 23767421
Submission date Jul 23, 2012
Last update date May 15, 2019
Contact name Yatendra Kumar
E-mail(s) [email protected], [email protected]
Organization name MRC Human Genetics Unit, The University Of Edinburgh
Department MRC HGU
Lab Wendy Bickmore
Street address Crewe Road south
City Edinburgh
ZIP/Postal code Eh42xu
Country United Kingdom
 
Platforms (1)
GPL9377 Illumina Genome Analyzer II (Saccharomyces cerevisiae)
Samples (6)
GSM971757 RPC128-FLAG IP_Normal Growth_Replicate1
GSM971758 RPC128-FLAG IP_Normal Growth_Replicate2
GSM971759 RPC128-FLAG MOCK_Normal Growth
Relations
BioProject PRJNA171220
SRA SRP014553

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39566_RAW.tar 458.3 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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