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| Status |
Public on Sep 28, 2012 |
| Title |
Differential methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis |
| Organism |
Trichinella spiralis |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We present the first study to confirm the existence of DNA methylation in the parasitic nematode Trichinella spiralis, and we characterize the methylomes of the three life-cycle stages of this food-borne infectious human pathogen. We observe a drastic increase in DNA methylation during the transition from the new born to mature stage, and we further identify parasitism-related genes that show changes in DNA methylation status between life cycle stages. We also evaluated differential gene expression among the three life stages using Illumina HiSeq 2000 RNA-seq technology.
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| |
|
| Overall design |
Examination of DNA methylation status and gene expression status in three life stages (Adult, muscle larvae, newborn larvae) of Trchinella Spiralis using MethylC-seq.
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| |
|
| Contributor(s) |
Liu M, Gao F |
| Citation(s) |
23075480 |
| |
| Submission date |
Jul 13, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Mingyuan Liu |
| E-mail(s) |
[email protected]
|
| Phone |
+86 13019125996
|
| Fax |
+86 431 87836702
|
| URL |
http://www.jluhp.edu.cn/zuzhijigou/yanjiusuo/yjs-ld/lmy.htm
|
| Organization name |
Jilin University
|
| Department |
Institute of Zoonosis
|
| Lab |
Key Laboratory for Zoonosis Research, Ministry of Education
|
| Street address |
5333 Xian Road
|
| City |
Changchun |
| State/province |
Jilin |
| ZIP/Postal code |
130062 |
| Country |
China |
| |
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| Platforms (2) |
| GPL15773 |
Illumina HiSeq 2000 (Trichinella spiralis) |
| GPL15806 |
Illumina Genome Analyzer (Trichinella spiralis) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA170655 |
| SRA |
SRP014316 |
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