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| Status |
Public on Dec 24, 2005 |
| Title |
Coordinated regulation of gene expression by Brn3a in developing sensory ganglia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Mice lacking the POU-domain transcription factor Brn3a exhibit marked defects in sensory axon growth and abnormal sensory apoptosis. We have determined the regulatory targets of Brn3a in the developing trigeminal ganglion using microarray analysis of Brn3a mutant mice. These results show that Brn3 mediates the coordinated expression of neurotransmitter systems, ion channels, structural components of axons and inter- and intracellular signaling systems. Loss of Brn3a also results in the ectopic expression of transcription factors normally detected in earlier developmental stages and in other areas of the nervous system. Target gene expression is normal in heterozygous mice, consistent with prior work showing that autoregulation by Brn3a results in gene dosage compensation. Detailed examination of the expression of several of these downstream genes reveals that the regulatory role of Brn3a in the trigeminal ganglion appears to be conserved in more posterior sensory ganglia but not in the CNS neurons that express this factor.
Keywords: Gene expression in the developing nervous system
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| Overall design |
Microarrays used to compare the patterns of gene expression in the trigeminal ganglia of Brn3a knockout and wild-type mice. Embryonic day 13.5 (E13.5) was chosen because at this point in development mutant mice exhibit major defects in sensory axon growth, but have yet to undergo the period of extensive sensory neuron death associated with later stages.
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| |
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| Contributor(s) |
Eng SR, Lanier J, Natalia F, Turner EE |
| Citation(s) |
15253936 |
| |
| Submission date |
Dec 22, 2005 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Eric Turner |
| E-mail(s) |
[email protected]
|
| Phone |
(858)534-1568
|
| Fax |
(858)534-7653
|
| URL |
http://medicine.ucsd.edu/neuroembryologylab/
|
| Organization name |
University of California San Diego
|
| Department |
Psychiatry
|
| Street address |
9500 Gilman Dr., BSB 2077
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0603 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
|
| Samples (11)
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| GSM89133 |
Brn3a Heterozygote TG e13.5 U74Av2 1 |
| GSM89134 |
Brn3a Knockout TG e13.5 U74Av2 1 |
| GSM89135 |
Brn3a Wildtype TG e13.5 U74Av2 1 |
| GSM89136 |
Brn3a Heterozygote TG e13.5 U74Av2 2 |
| GSM89137 |
Brn3a Knockout TG e13.5 U74Av2 2 |
| GSM89138 |
Brn3a Wildtype TG e13.5 U74Av2 2 |
| GSM89139 |
Heterozygote TG e13.5 U74Bv21 |
| GSM89140 |
Brn3a Knockout TG e13.5 U74Bv2 1 |
| GSM89141 |
Brn3a Wildtype TG e13.5 U74Bv2 1 |
| GSM89142 |
Brn3a Heterozygote TG e13.5 U74Bv2 2 |
| GSM89143 |
Brn3a Knockout TG e13.5 U74Bv2 2 |
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| Relations |
| BioProject |
PRJNA94143 |
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