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| Status |
Public on May 02, 2012 |
| Title |
Expression data from control and Ascorbic Acid (AA) stimulated Mc-3T3-E1 osteoblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Despite advances in investigating functional aspects of osteoblast (OB) differentiation, especially studies on how bone proteins are deposited and mineralized, there has been little research on the intracellular trafficking of bone proteins during OB differentiation. Collagen synthesis and secretion is markedly upregulated upon Ascorbic Acid (AA) stimulation. Understanding the mechanism by which collagen is mobilized in specialized OB cells is important for both basic cell biology and diseases involving defects in bone secretion and deposition. RabGTPases are major regulators on protein trafficking throughout the cell. In this study, we identified the Rab GTPases that are upregulated during 5-day AA differentiation of OBs using microarray analysis, namely Rab1, Rab3d and Rab27b.
We used microarrays to detail the global programme of gene expression underlying procollagen production and trafficking and identified up-regulated genes during this process.
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| Overall design |
Control Mc3T3-E1 cells and 5 day Ascorbic Acid stimulated cells were processed for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Nabavi N, Harrison RE |
| Citation(s) |
23050002, 23740245 |
| |
| Submission date |
Apr 30, 2012 |
| Last update date |
Sep 17, 2020 |
| Contact name |
Noushin Nabavi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Toronto
|
| Street address |
1265 Military Trail
|
| City |
Toronto |
| ZIP/Postal code |
M1C 1A4 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA162555 |
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