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| Status |
Public on Mar 06, 2012 |
| Title |
Differential expression of miRNA in stretched rat alveolar epithelial cells |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Cyclic stretch of alveoli is characteristic of mechanical ventilation, and is postulated to be partly responsible for the lung injury and inflammation in ventilator induced lung injury. We propose that miRNAs may regulate some of the stretch response and, therefore, hypothesized that miRNAs would be differentially expressed between stretched and unstretched rat alveolar epithelial cells (RAECs).
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| Overall design |
RAECs were isolated and cultured to express type I epithelial characteristics, after which they were equibiaxially stretched to 25% change in surface area at 15 cycles/minute for 1 or 6 hrs, or served as unstretched controls, and miRNA were extracted (n = 4 for all groups).
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| Contributor(s) |
Yehya N, Yerrapureddy A, Tobias J, Margulies SS |
| Citation(s) |
22537220 |
| |
| Submission date |
Mar 04, 2012 |
| Last update date |
Jan 13, 2016 |
| Contact name |
Nadir Yehya |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pennsylvania
|
| Department |
Bioengineering
|
| Street address |
390 Towne Bldg, U Penn
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL7722 |
miRCURY LNA microRNA Array, v.10.0 - hsa, mmu & rno |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA153075 |
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