|
| Status |
Public on Oct 20, 2005 |
| Title |
prp43 COLD SHIFT |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Cold senstive alleles of prp43, S249A and G429A were assayed for splicing and RNA processing defects.
Keywords: splicing, pre-mRNA processing
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| |
|
| Overall design |
RNA was isolated from cold senstive alleles of prp43, S249A and G429A after cold shift to 16C for 60 mins. Cy3/Cy5 labeled RNA's were hybridized to Yeast Splicing Chip v62 using WT RNA cold shifted to 16C for 60 mins as a reference. There are 4 hybes in the series which include reciprocal dye swap experiements for each cold sensitive allele.
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| |
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| Contributor(s) |
Nagel RJ |
| Citation(s) |
16382144 |
| |
| Submission date |
Oct 18, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Roland J Nagel |
| E-mail(s) |
nagel@darwin
|
| Phone |
831 425 5309
|
| Organization name |
UCSC
|
| Department |
Biology
|
| Lab |
ARES Lab
|
| Street address |
Sinsheimer Labs
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA93421 |
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