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| Status |
Public on Jan 01, 2012 |
| Title |
ChIP-on-chip analysis of H3K27me3 levels in leaves and calli |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
In plants, multiple detached tissues are capable of forming a pluripotent cell mass, termed callus, when cultured with appropriate plant hormones. Recent studies demonstrated that callus resembles the tip of a root meristem, even if it is derived from aerial organs. However, the underlying mechanism that guides differentiated organs to form callus is unknown. Here we show that genome-wide reprogramming of histone H3 lysine 27 trimethylation (H3K27me3) is critical in callus formation. During culture of leaf explants, the H3K27me3 level was decreased first at certain auxin-pathway genes, and then increased at the leaf- but decreased at the root-preferentially expressed genes. In addition, only the root but not the leaf explants of the Polycomb group (PcG) mutants can normally form callus. Our data indicate that PcG and H3K27me3 demethylation pathways act separately in reprogramming of H2K27me3 distributions, and also suggest that this is a general mechanism leading to cell fate transition.
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| Overall design |
We analyzed the genome-wide profiles by the ChIP-on-chip assay to determine genes with altered H3K27me3 modifications between the leaf and the callus
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| Contributor(s) |
Xu L, He C |
| Citation(s) |
22927830 |
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| Submission date |
Dec 21, 2011 |
| Last update date |
May 09, 2017 |
| Contact name |
Lin XU |
| E-mail(s) |
[email protected]
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| Phone |
86-21-54924101
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| Organization name |
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences
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| Department |
Chinese Academy of Sciences
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| Street address |
300 Fenglin Road
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA150481 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34596_RAW.tar |
204.4 Mb |
(http)(custom) |
TAR (of BAR, CEL) |
| GSE34596_decreased_H3K27me3_leaf-callus_transition_peak_1.ma.bar.gz |
18.0 Mb |
(ftp)(http) |
BAR |
| GSE34596_increased_H3K27me3_callus-leaf_transition_peak_1.ma.bar.gz |
18.0 Mb |
(ftp)(http) |
BAR |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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