|
| Status |
Public on Dec 01, 2005 |
| Title |
Carbon fixation (endogenous sugar) and light-dependent gene expression |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Internal sugar and light specific dependent regulation of leaf gene expression was addressed by changing [CO2] to lower than compensation point [CO2] in combination with light or prolonged darkness. Plants were grown on soil in a 12/12 h light/dark rhythm at 20°C day and night and under normal [CO2]. 5 weeks after germination, the above-ground rosettes of the non-flowering plants were harvested, 12 plants per sample. Plants were harvested 4hrs after the end of night (i) under low (< 50 ppm) [CO2] and 150 µE fluorescent light , (ii) under normal [CO2] and light, and, (iii) under low [CO2] and prolonged darkness. The low [CO2] treatment started 30 min before the end of night and stopped with harvesting.
Keywords: repeat
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| |
|
| Overall design |
2 biological replicates of plants under 3 treatments (4hrs low CO2+light, 4hrs low CO2+prolonged darkness, and 4hrs normal CO2+light).
|
| Web link |
http://www.mpimp-golm.mpg.de
|
| |
|
| Contributor(s) |
Bläsing OE, Stitt M |
| Citation missing |
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| |
| Submission date |
Oct 07, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Oliver E Bläsing |
| E-mail(s) |
[email protected]
|
| Phone |
+49 (0) 331 5678114
|
| Fax |
+49 (0) 331 5678 134
|
| Organization name |
Max-Planck-Institut Molekulare Pflanzenphysiologie
|
| Department |
Abt. Stitt
|
| Lab |
Stitt
|
| Street address |
Am Mühlenberg 1
|
| City |
Potsdam-Golm |
| ZIP/Postal code |
D-14476 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (6)
|
| GSM77175 |
04h low CO2 + light replicate A |
| GSM77176 |
04h normal CO2 + light replicate A |
| GSM77177 |
04h low CO2 + dark replicate A |
| GSM77178 |
04h low CO2 + light replicate B |
| GSM77179 |
04h normal CO2 + light replicate B |
| GSM77180 |
04h low CO2 + dark replicate B |
|
| Relations |
| BioProject |
PRJNA93455 |
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