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Series GSE33675 Query DataSets for GSE33675
Status Public on Jan 01, 2012
Title Role of miRNAs in the differentiation of human embryonic stem cells to an endothelial cell lineage
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To assess changes in the miRNA-ome during the early stages of endothelial differentiation, the expression of mature human miRNAs at days 2, 4 and 10 of endothelial differentiation (vs pluripotent time-matched samples) in SA461 hESC cell line was analysed in a two-channel microarray. Expression of the pluripotency-associated miRNAs miR-302a – d and miR-372 and miR-373 was significantly suppressed with progression of differentiation. The expression of miR-99b, miR-181a and miR-181b was, in contrast, increased in a time- and differentiation-dependent manner compared to time-matched pluripotent samples. The expression profiles were validated and good concordance was observed between microarray, Q-PCR and Northern blot analysis.
 
Overall design This experiment includes a total of 28 samples which are divided in to 3 groups, each with 4 biological repeats: group 1, Parental pluripotent hESC control sample. group 2, time-matched pluripotent hESC control samples. Group 3, hESC cells directed toward endothelial differentiation at time points 2 days, 4 days and 10 days.
 
Contributor(s) Kane NM, McClure JD, Baker AH
Citation(s) 22232059
Submission date Nov 14, 2011
Last update date May 01, 2012
Contact name Nicole Margaret Kane
Organization name University College London
Street address 30 Guilford Street
City London
ZIP/Postal code WC1N 1EH
Country United Kingdom
 
Platforms (1)
GPL14799 LC_MRA-1001_miRHuman_12,0 (miRNA ID version)
Samples (28)
GSM832644 SA461 Parental pluripotent hESC control sample rep1
GSM832645 SA461 Parental pluripotent hESC control sample rep2
GSM832646 SA461 Parental pluripotent hESC control sample rep3
Relations
BioProject PRJNA148295

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33675_RAW.tar 2.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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