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| Status |
Public on Oct 13, 2011 |
| Title |
CP190-HB.BG3.LacZ_RNAi |
| Project |
modENCODE
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| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
modENCODE_submission_3668
This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: The goal of these experiments are to validate and confirm the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. To do this, we are using RNAi to deplete individual non-histone chromosomal proteins in Drosophila BG3 and S2 tissue culture cells, and then using antibodies to perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. Comparison of a protein factor's binding profiles before and after depletion will increase the confidence of our predictions.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
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| Overall design |
EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 6; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody CP190-HB (target is CP190); dsRNA (RNAi_reagent) Fly_LacZ_RNAi&oldid=39667
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| Web link |
http://www.modencode.org
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
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| Contributor(s) |
Karpen G, Elgin S, Gortchakov A, Shanower G, Tolstorukov M, Kharchenko P, Kuroda M, Pirrotta V, Park P, Minoda A, Riddle N, Schwartz Y, Alekseyenko A, Kennedy C |
| Citation(s) |
21177972, 22767387 |
| BioProject |
PRJNA63463 |
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| Submission date |
Oct 11, 2011 |
| Last update date |
Jul 08, 2015 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
[email protected]
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| Phone |
416-673-8579
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| Organization name |
Ontario Institute for Cancer Research
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| Lab |
modENCODE DCC
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| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
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| Platforms (1) |
| GPL6629 |
[DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array |
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| Samples (6)
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE32778_1290.Mvalues.bedgraph.gz |
41.0 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE32778_472.Mvalues.bedgraph.gz |
41.4 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE32778_705.Mvalues.bedgraph.gz |
41.6 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE32778_RAW.tar |
163.3 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE32778_repset.9274438.smoothedM.bedgraph.gz |
41.6 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE32778_repset.9274438.smoothedM.clusters.gff3.gz |
169.8 Kb |
(ftp)(http) |
GFF3 |
| Processed data are available on Series record |
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