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| Status |
Public on Apr 23, 2013 |
| Title |
Identification of SHORT VEGETATIVE PHASE putative targets through whole-genome expression analysis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
SVP is a key MADS-box transcription factor for Arabidopsis development since it acts both during vegetative and reproductive phases where it plays different roles probably by interacting with different partners to regulate specific sets of target genes. In fact, whereas SVP functions as a repressor of floral transition during the vegetative phase, it works as floral meristem gene during reproductive phase.
We studied the behavior of SVP during two distinct developmental phases: the vegetative and reproductive phase. The aim of these studies is to identify subsets of genes that are regulated by SVP by means of Arabidopsis Tiling 1.0R Arrays (Affymetrix) during the two distinct phases of development.
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| Overall design |
Arabidopsis thaliana seedlings and inflorescences were selected at successive stages of early development for RNA extraction and hybridization on Affymetrix microarrays. To evaluate the amount of SVP expression in the vegetative phase we used svp-41 single mutant and wild-type seedlings grown for 2 weeks in Short Day (SD) conditions (8 h light/16 h dark); for the reproductive phase we used wild-type and svp-41 agl24-2 ap1-12 triple mutant inflorescences grown for 2 weeks in SD conditions and then moved in (LD) conditions (16 h light/16 h dark). The inflorescences were collected at 2 weeks after bolting.
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| Contributor(s) |
Gregis V, Sessa A, Andrés F, Guerra R, Mateos J, Torti S, Pavesi G, Zambelli F, Prazzoli GM, Grini PE, Colombo L, Simonini S, Bjerkan KN, Coupland G, Kater MM |
| Citation(s) |
23759218 |
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| Submission date |
Sep 26, 2011 |
| Last update date |
May 09, 2017 |
| Contact name |
Fernando Andres |
| E-mail(s) |
[email protected]
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| Organization name |
Max Planck Institute for Plant Breeding Research
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| Department |
Plant Developmental Biology
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| Lab |
Prof George Coupland
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| Street address |
Carl von Linne 10
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| City |
Cologne |
| ZIP/Postal code |
50829 |
| Country |
Germany |
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| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA147927 |
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