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| Status |
Public on Aug 23, 2011 |
| Title |
Bacillus subtilis Δ7ECF Pxyl-sigV + xylose vs Δ7ECF Pxyl-sigV - xylose , 168 + lysozyme vs 168 - lysozyme |
| Platform organism |
Bacillus subtilis |
| Sample organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Expression profiling by array
|
| Summary |
To define the ECF sigma sigV - regulated genes during log growth phase in LB media under induction conditions for sigV
The seven extracytoplasmic function (ECF) sigma (σ) factors of Bacillus subtilis are broadly implicated in resistance to antibiotics and other cell envelope stressors mediated, in part, by regulation of cell envelope synthesis and modification enzymes. We here define the regulon of σV as including at least 20 operons many of which are also regulated by σM, σX, or σW. The σV regulon is strongly and specifically induced by lysozyme and this induction is key to the intrinsic resistance of B. subtilis to lysozyme. Strains with null mutations in either sigV or in all seven ECF σ factor genes (Δ7ECF) have essentially equal increases in sensitivity to lysozyme. Induction of σV in the Δ7ECF background restores lysozyme resistance, whereas induction of σM, σX or σW does not. Lysozyme resistance results from the ability of σV to activate the transcription of two operons: the autoregulated sigV-rsiV-oatA-yrhK operon and dltABCDE. Genetic analyses reveal that oatA and dlt are largely redundant with respect to lysozyme sensitivity: single mutants are not affected in lysozyme sensitivity whereas a double oatA dltA mutant is as sensitive as a sigV null strain. Moreover, the triple sigV oatA dltA mutant is no more sensitive than the oatA dltA double mutant, indicating that there are no other σV-dependent genes necessary for lysozyme resistance. Thus, σV confers lysozyme resistance by activation of two cell wall modification pathways: O-acetylation of peptidoglycan catalyzed by OatA and D-alanylation of teichoic acids by DltABCDE.
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| Overall design |
Strains Δ7Pxyl-sigV + xylose vs. Δ7Pxyl-sigV - xylose, 168 + lysozyme vs. 168 - lysozyme. Each experiment was conducted 6 times using three independent total RNA preparations (biologlical triplicates). For each paired comparison, one sample was labeled with Alexa Fluor 555 and the other was with Alexa Fluor 647. For each comparison, three replicates were performed with dyeswap with the same RNA preparation.
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| Contributor(s) |
Guariglia-Oropeza V, Helmann J |
| Citation(s) |
21926231 |
| |
| Submission date |
Aug 22, 2011 |
| Last update date |
Feb 20, 2014 |
| Contact name |
John D. Helmann |
| E-mail(s) |
[email protected]
|
| Phone |
607 255 6570
|
| Organization name |
Cornell University
|
| Department |
Microbiology
|
| Street address |
372 Wing Hall
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL7420 |
Sigma Genosys Panorama Bacillus subtilis Gene Array |
|
| Samples (12)
|
| GSM783322 |
delta7 P-sigV + xyl vs delta7 P-sigV - xyl rep1 |
| GSM783323 |
delta7 P-sigV + xyl vs delta7 P-sigV - xyl rep2 |
| GSM783324 |
delta7 P-sigV + xyl vs delta7 P-sigV - xyl rep3 |
| GSM783325 |
delta7 P-sigV + xyl vs delta7 P-sigV - xyl rep1 dyeswap |
| GSM783326 |
delta7 P-sigV + xyl vs delta7 P-sigV - xyl rep2 dyeswap |
| GSM783327 |
delta7 P-sigV + xyl vs delta7 P-sigV - xyl rep3 dyeswap |
| GSM783328 |
168 + lys vs 168 - lys rep1 |
| GSM783329 |
168 + lys vs 168 - lys rep2 |
| GSM783330 |
168 + lys vs 168 - lys rep3 |
| GSM783331 |
168 + lys vs 168 - lys rep1 dyeswap |
| GSM783332 |
168 + lys vs 168 - lys rep2 dyeswap |
| GSM783333 |
168 + lys vs 168 - lys rep3 dyeswap |
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| Relations |
| BioProject |
PRJNA145691 |
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