|
| Status |
Public on Nov 23, 2011 |
| Title |
Developmental stage-specific gene expression in the two-spotted spider mite (Tetranychus urticae) |
| Organism |
Tetranychus urticae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generated 77-bp Illumina reads from single messenger RNA libraries from four diverse developmental stages of the two-spotted spider mite to maximally capture the complement of transcribed sequences across development. Adult, nymphal, larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for transcriptome library preparation. Samples were a mix of males and females to capture male and female patterns of transcription, and were reared on beans (Phaseolus vulgaris cv California Red Kidney). The RNA-Seq data was used for validation of gene models predicted by EuGene, and to study patterns of gene expression across development.
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| |
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| Overall design |
Gene expression for spider mites from adult, nymph, larvae and embryonic developmental stages was examined (technical replicates were generated).
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| |
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| Contributor(s) |
Clark RM, Osborne EJ |
| Citation(s) |
22113690, 22393009, 23663308 |
| |
| Submission date |
Aug 18, 2011 |
| Last update date |
Sep 16, 2019 |
| Contact name |
Richard M Clark |
| Organization name |
University of Utah
|
| Department |
Department of Biology
|
| Lab |
Clark Laboratory
|
| Street address |
257 So. 1400 East, RM 204 SB
|
| City |
Salt Lake City |
| State/province |
Utah |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14176 |
Illumina Genome Analyzer II (Tetranychus urticae) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE32342 |
The genome of Tetranychus urticae reveals herbivorous pest adaptations |
|
| Relations |
| SRA |
SRP007866 |
| BioProject |
PRJNA154989 |
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