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| Status |
Public on Sep 09, 2011 |
| Title |
SNP array analysis of lung cancer NCI-H1395, diluted to different tumor cell concentrations |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
SNP genotyping by SNP array
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| Summary |
In vitro mix of DNA from patient-matched lung cancer and blood cell lines, representing 30,50,70,100% tumor cell tissue, analyzed on Affymetrix 6.0 arrays.
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| Overall design |
Lung cancer cell line H-1395 and patient-matched blood cell line BL1395 were obtained from ATCC and cultured according to their recommendations. DNA extraction was performed using the DNeasy Tissue Kit (Qiagen, Hilden, Germany). DNA from patient-matched Lung cancer and Blood cell lines NCI-H1395 and NCI-BL1395 were mixed to simulate tumor tissue with 30,50,70,100% cancer cells. The DNA ratio was adjusted to compensate for NCI-H1395 being nearly triploid. DNA was analyzed on Affymetrix SNP6.0 microarrays according to the manufacturer's protocol.
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| Contributor(s) |
Rasmussen M, Isaksson A |
| Citation(s) |
22023820 |
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| Submission date |
May 10, 2011 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Anders Isaksson |
| E-mail(s) |
[email protected]
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| Organization name |
Uppsala University
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| Street address |
Akademiska sjukhuset ing61 3tr
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| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6801 |
[GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array |
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| Samples (4)
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| GSM721892 |
Lung_Cancer_Cell_Line_NCI-H1395 |
| GSM721893 |
Lung_Cancer_Cell_Line_NCI-H1395 and Blood_Cell_Line_NCI-BL1395 |
| GSM721894 |
Lung_Cancer_Cell_Line_NCI-H1395 and Blood_Cell_Line_NCI-BL1396 |
| GSM721895 |
Lung_Cancer_Cell_Line_NCI-H1395 and Blood_Cell_Line_NCI-BL1397 |
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| Relations |
| BioProject |
PRJNA139979 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29172_RAW.tar |
290.5 Mb |
(http)(custom) |
TAR (of CEL, CNCHP, ZIP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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