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| Status |
Public on Nov 27, 2024 |
| Title |
Effect of Vitamin D Modulation on the TH2 Cell Transcriptomic Landscape |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This dataset profiles the transcriptional response of in vitro polarized TH2 cells treated with calcitriol (active vitamin D) and vehicle controls. RNA-seq was used to identify differentially expressed genes influenced by vitamin D signaling. This study provides insight into the molecular mechanisms through which vitamin D modulates immune responses in TH2 cells.
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| Overall design |
The experimental design investigates the transcriptional impact of calcitriol (active vitamin D) treatment in in vitro polarized TH2 cells.TH2 cells were treated with 100 nM calcitriol for 24 hours. Vehicle controls were exposed to 2% ethanol (v/v). Differential expression analysis was performed between the calcitriol-treated group (N=11) and the vehicle control group (N=11). RNA-seq was performed to quantify gene expression, followed by downstream differential expression and enrichment analyses to identify genes and pathways impacted by calcitriol treatment.
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| Contributor(s) |
Fangal VD, Kiliç A, Mirzakani H, Litonjua AA, Damey MB, Levy BD, Weiss ST |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 26, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Vrushali Dipak Fangal |
| E-mail(s) |
[email protected]
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| Organization name |
Harvard Medical School
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| Department |
Brigham and Women's Hospital
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| Lab |
Channing Division of Network Medicine
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| Street address |
181 Longwood Avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA1191048 |
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