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Series GSE281942 Query DataSets for GSE281942
Status Public on Nov 15, 2024
Title Bioenergetic programs of cancellous and cortical bone are distinct and differ with age and mechanical loading
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mechanical loading induces bone formation in young rodents, but mechanoresponsiveness is reduced with age. Glycolytic activity and mitochondrial dysfunction increase with age and may change bone mechanotransduction. To evaluate load-induced changes to bioenergetic activity in young and adult animals, we loaded the tibia of 10-wk and 26-wk female mice and examined transcriptomic responses at the mid-diaphysis, metaphyseal cortical shell, and cancellous core. Across all biological processes, oxidative phosphorylation and mitochondrial pathways were most often enriched with loading and had opposite enrichment in young and adult animals. Following loading, young animals had temporally-coordinated differential expression of mitochondrial-associated genes, with greatest expression at the mid-diaphysis. In adults, bioenergetic gene expression was lower compared to young animals.
 
Overall design Comparative gene expression profiling of RNA-seq data for bone samples from loaded limbs and contralateral control limbs of 10wk and 26 wk old female C57Bl6 mice. At various time points following tibial loading, the cancellous core and 2 cortical segments (metaphyseal cortical shell and mid-diaphysis) were collected. Differential expression was assessed at each cortical location at 1-h, 3-h, and 24-h following a single loading session. To evaluate long term time points, a final group received 5 consecutive days of loading with euthanasia on the eighth day following the first loading session.
 
Contributor(s) Chlebek C, McNeill T, Huang M, Raynor M, van der Meulen M
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Submission date Nov 14, 2024
Last update date Nov 15, 2024
Contact name Carolyn Chlebek
E-mail(s) [email protected]
Organization name Cornell University
Department Biomedical Engineering
Lab van der Meulen
Street address 110 Hoy Road
City Ithaca
State/province NY
ZIP/Postal code 14850
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (150)
GSM8633235 10wk_Cancellous_Loaded Limb _3h _Animal3hYA
GSM8633236 10wk_Cancellous_Contralateral Control Limb _3h _Animal3hYA
GSM8633237 10wk_Cancellous_Loaded Limb _24h _Animal24hYA
Relations
BioProject PRJNA1186219

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Supplementary file Size Download File type/resource
GSE281942_Normalized_Counts_Chlebek_Bioenergetics_paper_2.xlsx 28.4 Mb (ftp)(http) XLSX
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