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| Status |
Public on Apr 13, 2012 |
| Title |
Human monocyte-derived macrophages polarized by GM-CSF or M-CSF |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Identification of genes differentially expressed between human monocyte-macrophages generated in the presence of either GM-CSF (termed M1) or M-CSF (termed M2)
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| |
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| Overall design |
Human peripheral blood monocytes from three independent healthy donors (#1, #2 and #3) were isolated by anti-CD14-labeled magnetic microbeads. CD14+ monocytes were cultured for 7 days in RPMI 10% FCS containing either GM-CSF or M-CSF. Total RNA from each condition was extracted and purified using the RNeasy kit (Qiagen). Labelled RNA was used as hybridization probes on human Codelink Whole genome Bioarray. All experimental procedures were performed following manufacturer instructions. Microarrays were scanned with a GenePix 4000B (Axon Instruments) scanner. Scanned images and raw data were processed using the Codelink Expression Software.
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| |
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| Contributor(s) |
Corbi AL, Vega MA |
| Citation(s) |
21389328, 24639350, 26209622 |
| |
| Submission date |
Mar 07, 2011 |
| Last update date |
Oct 15, 2015 |
| Contact name |
Angel L Corbí |
| E-mail(s) |
[email protected]
|
| Phone |
34 918373112
|
| Organization name |
Consejo Superior de Investigaciones Científicas
|
| Department |
Molecular Microbiology and Infection Biology
|
| Lab |
Myeloid Cell Laboratory
|
| Street address |
Ramiro de Maeztu 9
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL11010 |
CodeLink Human Whole Genome Bioarray |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA138333 |
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