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Series GSE275862 Query DataSets for GSE275862
Status Public on Oct 30, 2024
Title Differentially expressed genes between WT and AtC3H3 OX, and WT and AtC3H26 OX
Organism Arabidopsis
Experiment type Expression profiling by high throughput sequencing
Summary Salinity is a pressing issue causing widespread crop loss, prompting plants to adapt through changes in gene expression. This study investigated the role of the non-tandem CCCH zinc finger protein gene AtC3H3 in Arabidopsis in response to salt stress. AtC3H3, a gene from the non-TZF gene family and known for its RNA-binding and ribonuclease activity, was found to be upregulated under osmotic stresses such as high salt and drought. When overexpressed in Arabidopsis, AtC3H3 led to increased tolerance to salt stress, not drought stress. qRT-PCR analysis showed that the expression of both well-known ABA-dependent salt stress-responsive genes, such as RAB18, RD29B, and RD22, and representative ABA-independent salt stress-responsive genes, such as DREB2A and DREB2B, was significantly higher in AtC3H3 OXs than WT under NaCl treatment, indicating its involvement in both ABA-dependent and ABA-independent signaling pathways. mRNA-Seq analysis using NaCl-treated WT and AtC3H3 OXs revealed no potential target mRNAs of the RNase function of AtC3H3, suggesting that the potential targets of AtC3H3 might be non-coding RNAs, not mRNAs. The study conclusively demonstrates that AtC3H3 plays a crucial role in salt stress tolerance by influencing the expression of salt stress-responsive genes. These findings have opened a new avenue for understanding plant stress mechanisms and suggest potential strategies for enhancing crop resilience to salinity.
 
Overall design Differential gene expression between WT and AtC3H3 OX, as well as between WT and AtC3H26 OX, was examined under both no treatment and NaCl treatment conditions at 10 days after germination under SD conditions (8-hour-light and 16-hour-dark cycle) on a whole-genome level.
 
Contributor(s) Seok H, Lee S, Moon Y
Citation(s) 39456724
Submission date Aug 28, 2024
Last update date Oct 31, 2024
Contact name Yong-Hwan Moon
E-mail(s) [email protected]
Phone +82515102592
Organization name Pusan National University
Department Molecular Biology
Lab Plant Molecular Genetics Lab
Street address Busandaehak-ro 63Beon-gil 2, Geumjeong-gu
City Busan
ZIP/Postal code 46241
Country South Korea
 
Platforms (1)
GPL27784 Illumina NovaSeq 6000 (Arabidopsis)
Samples (14)
GSM8486727 WT_10DAG_control_1_rep1
GSM8486728 WT_10DAG_control_1_rep2
GSM8486729 WT_10DAG_control_2_rep1
Relations
BioProject PRJNA1153388

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE275862_RAW.tar 3.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA

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