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GEO help: Mouse over screen elements for information. |
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Status |
Public on Sep 26, 2024 |
Title |
Inhibition of CDK8/CDK19 with the small molecule inhibitor SEL120-34A in prostate cancer cell lines (3’ tag DGE) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The Mediator complex is a multi-subunit protein that modulates gene expression on a genome-wide scale. MED12 and cyclin-dependent kinase 8 (CDK8) or its paralog CDK19 are components of its kinase module that regulate the proliferation of prostate cancer cells. In this study, we investigated how MED12 and CDK8/19 influence cancer-driven processes in prostate cancer cell lines, focusing on AR activity and enzalutamide resistance.
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Overall design |
To investigate if CDK8/CDK19 inhibition has similar effects as downregulation of MED12 in enzalutamide sensitive and resistant prostate cancer cells, we treated the cells with SEL120-34A and analyzed the response using high-throughput 3’ tag differential gene expression analysis.
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Contributor(s) |
Andofi C, Handle F, Culig Z |
Citation(s) |
39253786 |
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Submission date |
Jun 04, 2024 |
Last update date |
Sep 26, 2024 |
Contact name |
Florian Handle |
E-mail(s) |
[email protected]
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Organization name |
Medical University of Innsbruck
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Department |
Institute of Pathology, Neuropathology and Molecular Pathology
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Street address |
Müllerstrasse 44
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City |
Innsbruck |
ZIP/Postal code |
6020 |
Country |
Austria |
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Platforms (1) |
GPL34284 |
Illumina NovaSeq X Plus (Homo sapiens) |
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Samples (14)
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GSM8305853 |
LNCaP, CDKinh, rep1 |
GSM8305854 |
LNCaP, CDKinh, rep2 |
GSM8305855 |
LNCaP, CDKinh, rep3 |
GSM8305856 |
22Rv1, DMSO, rep1 |
GSM8305857 |
22Rv1, DMSO, rep2 |
GSM8305858 |
22Rv1, DMSO, rep3 |
GSM8305859 |
22Rv1, CDKinh, rep1 |
GSM8305860 |
22Rv1, CDKinh, rep2 |
GSM8305861 |
22Rv1, CDKinh, rep3 |
GSM8305862 |
PC3, DMSO, rep1 |
GSM8305863 |
PC3, CDKinh, rep1 |
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Relations |
BioProject |
PRJNA1120051 |
Supplementary file |
Size |
Download |
File type/resource |
GSE269071_count_file.txt.gz |
802.9 Kb |
(ftp)(http) |
TXT |
GSE269071_log2CPM_expression.txt.gz |
610.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
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