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| Status |
Public on Dec 01, 2024 |
| Title |
A novel, butyrate-producing Gram-positive bacterium from the human gut, Hoskinsella mucinolytica, selectively targets N-acetylhexosamine sugars in host mucins |
| Organism |
Erysipelotrichaceae bacterium DSM 116809 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used culturing of fecal sample enrichments on solid medium containing gastric mucin as the main carbon source to isolate a novel bacterium that is largely restricted to using the N-acetylglucosamine and N-acetylgalactosamine sugars from mucin. This butyrate-producing bacterium accesses these sugars from both polymeric gastric mucin and chemically released oligosaccharides and has a genome with correspondingly restricted carbohydrate-active enzyme content. Sequencing data was curated to determine gene expression profiles when comparing N-acetylgalactosamine, N-acetylglucosamine, gastric mucin oligosaccharides and cellobiose.
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| Overall design |
Hoskinsella mucinolytica DSM116809 was grown in biological replicate cultures to mid-log phase under anaerobic conditions at 37ºC on Depleted Medium (DM) containing either N-acetylgalactosamine, N-acetylglucosamine, gastric mucin oligosaccharides (gMO) or cellobiose. Total RNA was extracted using phenol:chloroform and rRNA was removed. Sequencing of mRNA (RNA-sequencing) analysis was performed to determine differential gene expression among these substrates.
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| Contributor(s) |
Pudlo NA, Pereira GV, Martens EC |
| Citation missing |
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| |
| Submission date |
May 16, 2024 |
| Last update date |
Dec 01, 2024 |
| Contact name |
Nicholas Pudlo |
| E-mail(s) |
[email protected]
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| Organization name |
University of Michigan
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| Street address |
1150 W. Medical Center Dr.
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| City |
Ann Arbor |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL34484 |
Illumina NovaSeq 6000 (Erysipelotrichaceae bacterium DSM 116809) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA1112426 |
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