|
| Status |
Public on May 15, 2012 |
| Title |
Gene expression profile of FOXM1 with Doxorubicin in MDA-MB-231 breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcription factors (TFs) play a crucial role in diverse cellular physiology by controlling down-stream target genes. It has been well recognized that TFs are attractive target for treating human cancer since transcriptional activity or gene expression level of TFs can be directly modulated. To explore which TF functions as survival factor in human breast cancer, we applied gene expression data sets for survival analysis. We identified that FOXM1 is a potent oncogenic survival factor out of eleven TFs. Loss of FOXM1 function leads to breast cancer cells more sensitized to Doxorubicin (Dox). To investigate how FOXM1 sensitizes cancer cell in the presence of Dox, we carried out gene expression array experiment to monitor how down-stream target genes are changed by FOXM1. MDA-MB-231 cells were incubated with siLuc or siFOXM1. Thirty six hours later, cells were incubated with Dox (5micro mol) for 36 hrs.
|
| |
|
| Overall design |
3 siLuc., 3 siFOXM1, 3 siLuc and Dox treatment, 3 siFOXM1 and Dox treatment
|
| |
|
| Contributor(s) |
Park Y, Kim S, Lee J |
| Citation(s) |
22581827 |
| |
| Submission date |
Dec 01, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Yun-Yong Park |
| E-mail(s) |
[email protected]
|
| Phone |
7137458525
|
| Fax |
7135634235
|
| URL |
http://www.mdanderson.org
|
| Organization name |
UT, MD Anderson Cancer Center
|
| Department |
Systems Biology
|
| Street address |
7435 Fannin St.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA134091 |
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