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| Status |
Public on Jun 19, 2024 |
| Title |
PRC2-AgeIndex: a universal biomarker of aging and rejuvenation [WGBS] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
DNA methylation (DNAm) is one of the most reliable biomarkers of aging across many mammalian tissues. While the age-dependent global loss of DNAm has been well characterized, age-dependent DNAm gain is less specified. Multiple studies have demonstrated that polycomb repressive complex 2 (PRC2) targets are enriched among the CpG sites which gain methylation with age. However, systematic whole-genome examination of all PRC2 targets in the context of aging methylome as well as determination of the pan-tissue or tissue-specific nature of these associations is lacking. Here, by analyzing DNAm data from different assays and from multiple young and old human and mouse tissues, we found that low-methylated regions (LMRs) which are highly bound by PRC2 in embryonic stem cells (PRC2 LMRs) gain methylation with age in all examined somatic mitotic cells. We also estimated that this epigenetic change represents around 90% of the age-dependent DNAm gain genome-wide. Therefore, we propose the “PRC2-AgeIndex,” defined as the average DNAm in PRC2 LMRs, as a universal biomarker of cellular aging in somatic cells. In addition, we demonstrate the application of this biomarker in the evaluation of different anti-aging interventions, including dietary restriction and partial epigenetic reprogramming.
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| Overall design |
WGBS data of neonatal and old fibroblasts from three different donors each.
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| Contributor(s) |
Moqri M, Cipriano A, Simpson DJ, Rasouli S, de Jong TA |
| Citation(s) |
39009581 |
| |
| Submission date |
Jan 23, 2024 |
| Last update date |
Aug 09, 2024 |
| Contact name |
Vittorio Sebastiano |
| E-mail(s) |
[email protected]
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| Organization name |
Stanford University
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| Department |
OB-GYN/Reproductive, Perinatal and Stem Cell Biology Research
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| Lab |
Sebastiano Lab
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| Street address |
265 Campus Drive
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305-5454 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE253987 |
PRC2-AgeIndex: a universal biomarker of aging and rejuvenation |
|
| Relations |
| BioProject |
PRJNA1068062 |
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