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| Status |
Public on Nov 05, 2024 |
| Title |
Transcriptional remodeling of human hematopoeitic stem cells in vitro by Interleukin-1β |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Trained immunity is a phenomenon where innate immune cells develop and long-lasting pro-inflammatory reprogramming mediated by epigenetic and metabolic reprogramming. Trained immunity can be peropheral (circulating monocytes) or central (bone marrow) and it can be caused by exposure to β-glucan, BCG and Western Type Diet vial IL-1β signaling.
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| Overall design |
HPCs were isolated from bone marrow mononuclear cells by CD34+ mangenitc separation. CD34+ cells were then exposed for 4 hours to 10 ng/ml IL1b. After incubation, cells were washed and seeded in 24-well plates at a density of 50,000 cells per well for expansion (10 days) and differentiation into monocytes for 7 days (see details in Growth protocol). At day 7 of differentiation, pan monocytes were magenetically separated. 500.000 cells per condition were then incubated for 4 hours with medium only or LPS 10 ng/ml; after the incubation time, the cells were washed and stored in RNAlater until further RNA extraction. RNA isolation was done using the QIAGEN AllPrep DNA/RNA micro-kit according to manufacturer’s instructions.
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| Web link |
https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(24)00266-2
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| Contributor(s) |
Flores-Gomez D, Kessler EL, Novakovic B, Riksen N, Bekkering S |
| Citation(s) |
39515317 |
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| Submission date |
Jan 19, 2024 |
| Last update date |
Nov 20, 2024 |
| Contact name |
Boris Novakovic |
| E-mail(s) |
[email protected]
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| Phone |
0434980073
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| Organization name |
Murdoch Children's Research Institute
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| Street address |
Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road
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| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1066945 |
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