|
| Status |
Public on Jan 18, 2024 |
| Title |
Activating transcription factors Atf3/Atf4 drive early molecular events preceding neural stem cell differentiation and control neuron formation [Short] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The generation of new neurons from neural stem cells is tightly controlled by transcriptional programs. To induce cell fate switches that establish neuronal identities, differentiating neural precursor cells (NPCs) must undergo rapid and dynamic gene expression changes. However, the neuronal fate-determining molecular events that precede NPC differentiation are incompletely understood. Using high temporal resolution transcriptional profiling of adult hippocampal NPC differentiation, we here identified the activating transcription factors Atf3 and Atf4 as early switch regulators that drive NPC fate and control neuron formation. We found that Atf3/4 are rapidly upregulated in NPCs upon induction of differentiation and drive dynamic gene expression changes associated with neurogenesis. Pharmacological and genetic perturbation of Atf4 demonstrated its importance for neuron formation. Sustained overexpression of Atf3 hampered neurogenesis by repressing long non-coding RNA Miat. Our results demonstrate the critical role of an Atf3/Atf4-controlled transcriptional network in driving early molecular events during neurogenesis.
|
| |
|
| Overall design |
17 samples from different timepoints after onset of differentiation
|
| |
|
| Contributor(s) |
Overall RW, Claasen J, Patone G, Hübner N, Kempermann G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 18, 2024 |
| Last update date |
Jan 19, 2024 |
| Contact name |
Rupert W Overall |
| Organization name |
Humboldt University of Berlin
|
| Street address |
Philippstraße 13
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL17543 |
Illumina MouseWG-6 v2.0 R2 expression beadchip |
|
| Samples (17)
|
|
| This SubSeries is part of SuperSeries: |
| GSE253534 |
Activating transcription factors Atf3/Atf4 drive early molecular events preceding neural stem cell differentiation and control neuron formation |
|
| Relations |
| BioProject |
PRJNA1066225 |
Less...
More...