 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 30, 2024 |
| Title |
RNA-seq of danshen injection, astaxanthin and diosmetin for the treatment of anthracycline-induced cardiotoxicity in H9c2 cardiomyocytes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Doxorubicin (DOX) and other anthracyclines are effective chemotherapeutic agents, however, their use is influenced by the risk of cardiotoxicity. We still have an incomplete understanding of the cardiomyocyte protective pathways activated after anthracycline-induced cardiotoxicity (AIC).Danshen injection (DSI), astaxanthin (AXT) and diosmetin (DMT) are effective in the treatment of cardiovascular diseases, but the mechanism of protection against adriamycin-induced cardiotoxicity is unclear. Here, we performed RNA-seq screening in H9c2 cardiomyocytes to determine the potential protective mechanisms of Danshen injection, astaxanthin and diosmetin against AIC.
|
| |
|
| Overall design |
To investigate the therapeutic effects of danshen injection, astaxanthin and diosmetin, respectively, in anthracycline-induced cardiotoxicity, we divided cultured H9c2 cells into five groups:a control group treated with PBS, a model group exposed to 2μM DOX, a treatment group subjected to 2μM DOX in combination with 50μL DSI diluted 1/20 in culture volume, a treatment group subjected to 2μM DOX in combination with 5μM AXT and a treatment group subjected to 2μM DOX in combination with 10μM DMT. Each group consisted of three samples, with DOX added after 4 hours of DSI, AXT an DMT treatment. The cells were harvested 24 hours after DOX treatment, then we performed gene expression profiling analysis using data obtained from RNA-seq. Comparative gene expression profiling analysis of RNA-seq data.
|
| |
|
| Contributor(s) |
Wan G, Zhu X, Pan Y, Chen M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 30, 2023 |
| Last update date |
Oct 30, 2024 |
| Contact name |
yijun pan |
| E-mail(s) |
[email protected]
|
| Phone |
17807206056
|
| Organization name |
湖北医药学院
|
| Street address |
湖北省十堰市人民南路30号
|
| City |
湖北襄阳 |
| State/province |
--请选择-- |
| ZIP/Postal code |
442000 |
| Country |
China |
| |
|
| Platforms (2) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA1033607 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE246548_processed_data.xlsx |
2.9 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...