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| Status |
Public on Oct 05, 2023 |
| Title |
Gene expression profiles at the single cell levels of murine airway T helper cells challenged with dog allergens |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We established a mouse model of dog allergy by repeatedly administering dog dander and epithelium extracts via the intranasal route. scRNA-Seq analysis of T helper cells in the airways pinpointed a unique gene signature for TH17 cells. Analysis of T-cell receptors depicted a high frequency of clones that were shared between TH17, TH2 and suppressive Treg cells, indicative of a common differentiation trajectory for these subsets.
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| Overall design |
Using WT challenged with dog allergens we performed single cell RNA-sequencing (scRNA-SEQ) using the 10X platform on airway-infiltrating CD4+ T helper cells.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/35657107/
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| Contributor(s) |
Tibbitt CA, Coquet JM |
| Citation(s) |
35657107 |
| |
| Submission date |
Oct 05, 2023 |
| Last update date |
Oct 11, 2023 |
| Contact name |
Chris Andrew Tibbitt |
| E-mail(s) |
[email protected]
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| Organization name |
KI
|
| Street address |
Biomedicum C7,, Tomtebodavägen 16
|
| City |
Solna |
| State/province |
non-US |
| ZIP/Postal code |
171 71 |
| Country |
Sweden |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
| GSM7825030 |
Airway T helper cells from dog allergen challenged mice |
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| Relations |
| BioProject |
PRJNA1024448 |
