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Series GSE241754 Query DataSets for GSE241754
Status Public on Oct 15, 2024
Title Dynamics of the zebrafish tRNAome during the maternal-to-zygotic transition [tRNA-Seq]
Organism Danio rerio
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Time course analysis of tRNA abundance during zebrafish early embryonic development.
 
Overall design Wild-type (TLAB strain) zebrafish embryos were grown in standard housing conditions.Unfertilized eggs (0 hours post-fertilization; hpf) were collected or embryos were staged and collected at consecutive developmental time-points, namelyat the 256-cell (2.5 hpf), 1000-cell (3 hpf), sphere (4 hpf), shield (6 hpf) and bud (10 hpf) stages. Eggs and embryos were either flash-frozen in liquid nitrogen or immediately processed. Samples were used for western blotting analysis, polysome profiling, ribosome profiling or mRNA and tRNA sequencing for investigating the regulation of tRNA gene expression and translational status during early zebrafish embryogenesis.
 
Contributor(s) Reimão-Pinto MM, Behrens A, Forcelloni S, Fröhlich K, Kaya S, Nedialkova DD
Citation(s) 39402326
Submission date Aug 28, 2023
Last update date Jan 17, 2025
Contact name Danny Nedialkova
Organization name Max Planck Institute of Biochemistry
Street address Am Klopferspitz 18
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platforms (2)
GPL20828 Illumina NextSeq 500 (Danio rerio)
GPL24995 Illumina NovaSeq 6000 (Danio rerio)
Samples (18)
GSM7734771 WT egg (0 hpf) tRNA-seq rep1
GSM7734772 WT egg (0 hpf) tRNA-seq rep2
GSM7734773 WT 256-cell (2.5 hpf) tRNA-seq rep1
This SubSeries is part of SuperSeries:
GSE241755 Dynamics of the zebrafish tRNAome during the maternal-to-zygotic transition
Relations
BioProject PRJNA1009808

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241754_cca_chargeRatio.csv.gz 2.6 Kb (ftp)(http) CSV
GSE241754_mimtRNAseq_transcriptCounts.csv.gz 46.3 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA

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