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| Status |
Public on Apr 01, 2011 |
| Title |
Finding direct target genes of VND7 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The Arabidopsis thaliana NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), acts as a key regulator of xylem vessel differentiation. In order to identify direct target genes of VND7, we performed global transcriptome analysis using Arabidopsis transgenic lines in which VND7 activity could be induced post-translationally. This analysis identified 63 putative direct target genes of VND7, which encode a broad range of proteins, such as transcription factors, IRREGULAR XYLEM proteins and proteolytic enzymes, known to be closely associated with xylem vessel formation. Recombinant VND7 protein binds to several promoter sequences present in candidate direct target genes: specifically, in the promoter of XYLEM CYSTEINE PEPTIDASE1, two distinct regions were demonstrated to be responsible for VND7 binding. We also found that expression of VND7 restores secondary cell wall formation in the fiber cells of inflorescence stems of nst1 nst3 double mutants, as well as expression of NAC SECONDARY WALL THICKENING PROMOTING FACTOR3 (NST3, however, the vessel-type secondary wall deposition was observed only as a result of VND7 expression. These findings indicated that VND7 upregulates, directly and/or indirectly, many genes involved in a wide range of processes in xylem vessel differentiation, and that its target genes are partially different from those of NSTs.
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| Overall design |
Total RNAs of the transgenic plants expressing 35S:VND7-VP16-GR treated with DEX plus CHX and those treated with CHX only were compared. As a control experiment, transgenic plants harboring empty vector were treated similarly and the total RNAs were compared similarly to identify genes merely induced by DEX treatment itself.
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| Contributor(s) |
Yamaguchi M, Ohtani M, Demura T, Mitsuda N |
| Citation(s) |
21284754 |
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| Submission date |
Sep 16, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
[email protected]
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| Organization name |
National Institute of Advanced Industrial Science and Technology
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| Department |
Bioproduction Research Institute
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| Lab |
Gene Regulation Research Group
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| Street address |
Central 6 Higashi 1-1-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (20)
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| GSM594701 |
10d seedlings expressing 35S:VND7-VP16-GR treated with DEX plus CHX, rep1 |
| GSM594702 |
10d seedlings expressing 35S:VND7-VP16-GR treated with DEX plus CHX, rep2 |
| GSM594703 |
10d seedlings expressing 35S:VND7-VP16-GR treated with DEX plus CHX, rep3 |
| GSM594704 |
10d seedlings expressing 35S:VND7-VP16-GR treated with DEX plus CHX, rep4 |
| GSM594705 |
10d seedlings expressing 35S:VND7-VP16-GR treated with DEX plus CHX, rep5 |
| GSM594706 |
10d seedlings expressing 35S:VND7-VP16-GR treated with CHX, rep1 |
| GSM594707 |
10d seedlings expressing 35S:VND7-VP16-GR treated with CHX, rep2 |
| GSM594708 |
10d seedlings expressing 35S:VND7-VP16-GR treated with CHX, rep3 |
| GSM594709 |
10d seedlings expressing 35S:VND7-VP16-GR treated with CHX, rep4 |
| GSM594710 |
10d seedlings expressing 35S:VND7-VP16-GR treated with CHX, rep5 |
| GSM594711 |
10d seedlings harboring empty vector treated with DEX plus CHX, rep1 |
| GSM594712 |
10d seedlings harboring empty vector treated with DEX plus CHX, rep2 |
| GSM594713 |
10d seedlings harboring empty vector treated with DEX plus CHX, rep3 |
| GSM594714 |
10d seedlings harboring empty vector treated with DEX plus CHX, rep4 |
| GSM594715 |
10d seedlings harboring empty vector treated with DEX plus CHX, rep5 |
| GSM594716 |
10d seedlings harboring empty vector treated with CHX, rep1 |
| GSM594717 |
10d seedlings harboring empty vector treated with CHX, rep2 |
| GSM594718 |
10d seedlings harboring empty vector treated with CHX, rep3 |
| GSM594719 |
10d seedlings harboring empty vector treated with CHX, rep4 |
| GSM594720 |
10d seedlings harboring empty vector treated with CHX, rep5 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA130089 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24169_RAW.tar |
42.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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