|
| Status |
Public on Sep 07, 2012 |
| Title |
Transcriptomic profiles of six strains of Lactococcus lactis in ultrafiltration-cheese model |
| Organism |
Lactococcus lactis |
| Experiment type |
Expression profiling by array
|
| Summary |
The intra sub-species diversity of six strains of Lactococcus lactis subsp. lactis was investigated at the genomic level and in terms of phenotypic and transcriptomic profiles in UF-cheese model. Six strains were isolated from various sources, but all are exhibiting a dairy phenotype. Our results showed that, the six strains exhibited small phenotypic differences since similar behaviour in terms of growth was obtained during cheese ripening while only different acidification capability was detected. Even if all strains displayed high genomic similarities, sharing a high core genome of almost two thousands genes, the expression of this core genome directly in the cheese matrix revealed major strain-specific differences. This strains with the same dairy origin.
|
| |
|
| Overall design |
Strains were cultured on skimmed raw milk ultrafiltration (UF) retentate. The UF retentate was pre-incubated overnight at 4 °C, then 45 minutes at 50 °C and homogenized during 1.5 minutes at 24 000 rpm with an ultra-turax (Imlab, France). After addition of rennet (0.3 µl ml-1), 400 g UF retentate was inoculated at 2 106 CFU/g with L. lactis subsp. lactis strains. After incubation for 8 hours at 30 °C, the cheeses were transferred at 12° C until 7 days for ripening simulation. At least three independent cultures of the six strains were performed. Total RNA was extracted from cells grown 24 hours in UF-cheese and radiolabelled cDNA were prepared and hybridized on nylon arrays. 1948 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. 3 independent repetitions were performed.
|
| |
|
| Contributor(s) |
Tan-a-ram P, Cardoso T, Daveran-Mingot M, Kanchanatawee S, Loubiere P, Girbal L, Cocaign-Bousquet M |
| Citation(s) |
21131529 |
| |
| Submission date |
Sep 07, 2010 |
| Last update date |
Sep 07, 2012 |
| Contact name |
Muriel Cocaign-Bousquet |
| E-mail(s) |
[email protected]
|
| URL |
http://www.insa-toulouse.fr/fr/recherche/labo/lisbp.html
|
| Organization name |
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
|
| Department |
UMR5504 INSA/CNRS - UMR792 INSA/INRA
|
| Street address |
135 avenue de Rangueil
|
| City |
Toulouse |
| ZIP/Postal code |
31077 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL6414 |
INSA Lactococcus lactis IL1403 (lot de PCR n°2) |
|
| Samples (18)
|
| GSM590777 |
L. lactis UCMA5713 Transcriptome cheese replicate 1 |
| GSM590778 |
L. lactis UCMA5713 Transcriptome cheese replicate 2 |
| GSM590779 |
L. lactis UCMA5713 Transcriptome cheese replicate 3 |
| GSM590780 |
L. lactis LD55 Transcriptome cheese replicate 1 |
| GSM590781 |
L. lactis LD55 Transcriptome cheese replicate 2 |
| GSM590782 |
L. lactis LD55 Transcriptome cheese replicate 3 |
| GSM590783 |
L. lactis LD61 Transcriptome cheese replicate 1 |
| GSM590784 |
L. lactis LD61 Transcriptome cheese replicate 2 |
| GSM590785 |
L. lactis LD61 Transcriptome cheese replicate 3 |
| GSM590786 |
L. lactis LL08 Transcriptome cheese replicate 1 |
| GSM590787 |
L. lactis LL08 Transcriptome cheese replicate 2 |
| GSM590788 |
L. lactis LL08 Transcriptome cheese replicate 3 |
| GSM590789 |
L. lactis LL52 Transcriptome cheese replicate 1 |
| GSM590790 |
L. lactis LL52 Transcriptome cheese replicate 2 |
| GSM590791 |
L. lactis LL52 Transcriptome cheese replicate 3 |
| GSM590792 |
L. lactis S86 Transcriptome cheese replicate 1 |
| GSM590793 |
L. lactis S86 Transcriptome cheese replicate 2 |
| GSM590794 |
L. lactis S86 Transcriptome cheese replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE24015 |
Genomic and transcriptomic analyses of Lactococcus lactis strains |
|
| Relations |
| BioProject |
PRJNA133193 |
Less...
More...