 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 13, 2023 |
| Title |
NGFR regulates germinal center B-cell activation and negative selection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The expression of the Nerve growth factor receptor (NGFR) was described in follicular dendritic cells (FDCs), the major lymphoid stromal cell (LSC) compartment regulating B-cell activation within germinal centers (GCs). However, the role of NGFR in humoral response is not well defined. In this work, we have studied the effect of Ngfr KO in LNs organization and function. Ngfr KO led to spontaneous GC formation and expansion of GC B-cell compartment that were related on Ngfr depletion in non-hematopoietic radioresistant compartment. In agreement, Ngfr KO mice showed alterations in LSC with an increased frequency of FDCs harboring an activated phenotype characterized by the overexpression of CD21/35, MAdCAM-1, and VCAM-1. Moreover, Ngfr KO mice showed GC ectopic location, loss of polarization, impaired high-affinity antibody production, and increased circulating autoantibodies. In addition, Ngfr KO/Bcl2 Tg mice displayed increased levels of autoantibodies, higher incidence of autoimmunity, and decreased overall survival. Our work shows that NGFR maintains GC structure and functionality being involved in the regulation of antibody production and immune tolerance.
|
| |
|
| Overall design |
To investigate differences in gene expression profiles we isolated FDCs from WT and Ngfr KO popliteal lymph nodes by FACS. Lymph nodes from several animals were pooled for each sample.
Gene expression profiling was performed using 4 WT and 3 Ngfr KO independent samples
|
| |
|
| Contributor(s) |
Hernández-Barranco A, Benguría A, Vazquez E, Dopazo A, Peinado H |
| Citation(s) |
38307025 |
| |
| Submission date |
Jul 05, 2023 |
| Last update date |
Mar 14, 2024 |
| Contact name |
Alberto Hernández-Barracno |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Spanish National Cancer Research Center
|
| Department |
Molecular Oncology
|
| Lab |
Microenvironment and Metastasis
|
| Street address |
Melchor Fernandez Almagro 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA991508 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE236511_TMMs.txt.gz |
788.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...