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| Status |
Public on May 16, 2024 |
| Title |
Defined conditions control the morphological dualism of rat primitive extraembryonic endoderm stem cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report two chemically defined conditions that differ by the addition of the cytokine Lif and the β-catenin-stabilizing drug Chir99021 and enable permanent self-renewal as mesenchymal and epithelial morphotypes, respectively. The morphotypes are interconvertible and differentiate equipotently. Surprisingly, the proliferation of both morphotypes requires Lif/Gp130/Stat3 signaling (autocrine in the absence of added Lif) and non-canonical Wnt signaling (autocrine). Additionally, the epithelial version requires β-catenin for proliferation and morphology. Interestingly, the mesenchymal cells also express key epithelial markers, but those are improperly structured and/or not functional, indicating a primed state. The results provide an improved platform for studying the proliferation and plasticity of the early extraembryonic endoderm, which occurs in mesenchymal and epithelial forms in vivo.
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| Overall design |
mRNA profiles of rat pXEN cells routinely cultured in T,LPE and CPE conditions were generated by deep sequencing, in triplicate, on an Illumina HiSeq 2500
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/37823730/
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| Contributor(s) |
Binas B, Chai YG |
| Citation(s) |
37823730 |
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| Submission date |
May 17, 2023 |
| Last update date |
Aug 15, 2024 |
| Contact name |
Young Gyu Chai |
| E-mail(s) |
[email protected]
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| Phone |
+82-31-400-5513
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| Organization name |
Hanyang Univ.
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| Department |
Molecualr & Life Science
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| Street address |
55 Hanyangdaehak-ro
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| City |
Ansan |
| State/province |
Gyeonggi-do |
| ZIP/Postal code |
15588 |
| Country |
South Korea |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA973697 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE232755_RAW.tar |
910.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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