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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 24, 2024 |
| Title |
RNA-seq and m6A-seq analysis of the control-iBAT and M14KO -iBAT, and RNA-seq analysis for the control-hBAT and M14KO-hBAT |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
m6A mRNA methylation in brown adipose tissue regulates systemic insulin sensitivity. We performed N6-methyladenosine (m6A) profiling and RNA seq of brown adipose tissue (BAT) from control and Mettl14-KO mice. Meanwhile, we performed RNA-seq for control and Mettl14-KO human BAT cell line as well.
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| Overall design |
The experiment was performed in homozygous, male control, and M14 KO mice. All mice were fed with chow-diet for twenty-four weeks from four week of age. At the end of the experiment, mice were euthanized after overnight fasting, followed by serum and tissue sample collection.
hBAT cells were differentiated for 21 days, washed with PBS and cultured in 1% BSA containing high-glucose DMEM medium for 24 hours, and then collected cell pellet for RNA extraction.
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| Web link |
https://doi.org/10.1016/j.cmet.2024.08.006
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| Contributor(s) |
Ju C, Xiao L, Kulkarni RN, He C |
| Citation(s) |
39255799 |
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| Submission date |
May 13, 2023 |
| Last update date |
Oct 24, 2024 |
| Contact name |
Cheng-Wei Ju |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Chicago
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| Lab |
Chuan He Lab
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| Street address |
929 East 57th Street, GCIS, Room E313
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| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (21)
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| GSM7339675 |
homozygous male mouse, Control, input, biol rep 1 |
| GSM7339676 |
homozygous male mouse, Control, input, biol rep 2 |
| GSM7339678 |
homozygous male mouse, Control, input, biol rep 3 |
| GSM7339679 |
homozygous male mouse, Control, input, biol rep 4 |
| GSM7339680 |
homozygous male mouse, Mettl14 KO, input, biol rep 1 |
| GSM7339681 |
homozygous male mouse, Mettl14 KO, input, biol rep 2 |
| GSM7339682 |
homozygous male mouse, Mettl14 KO, input, biol rep 3 |
| GSM7339683 |
homozygous male mouse, Mettl14 KO, input, biol rep 4 |
| GSM7339685 |
homozygous male mouse, Control, m6A IP, biol rep 1 |
| GSM7339686 |
homozygous male mouse, Control, m6A IP, biol rep 2 |
| GSM7339687 |
homozygous male mouse, Control, m6A IP, biol rep 3 |
| GSM7339688 |
homozygous male mouse, Control, m6A IP, biol rep 4 |
| GSM7339689 |
homozygous male mouse, Mettl14 KO, m6A IP, biol rep 1 |
| GSM7339690 |
homozygous male mouse, Mettl14 KO, m6A IP, biol rep 2 |
| GSM7339692 |
homozygous male mouse, Mettl14 KO, m6A IP, biol rep 3 |
| GSM7339693 |
homozygous male mouse, Mettl14 KO, m6A IP, biol rep 4 |
| GSM7339694 |
human brown adipocyte cell line, Control, biol rep 1 |
| GSM7339695 |
human brown adipocyte cell line, Control, biol rep 2 |
| GSM7339696 |
human brown adipocyte cell line, Control, biol rep 3 |
| GSM7339698 |
human brown adipocyte cell line, Mettl14 KO, biol rep 1 |
| GSM7339699 |
human brown adipocyte cell line, Mettl14 KO, biol rep 2 |
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| Relations |
| BioProject |
PRJNA972313 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE232491_hBAT_Deseq2.csv.gz |
2.2 Mb |
(ftp)(http) |
CSV |
| GSE232491_iBAT_Deseq2.csv.gz |
2.4 Mb |
(ftp)(http) |
CSV |
| GSE232491_iBAT_RADRA.csv.gz |
933.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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