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| Status |
Public on Sep 21, 2023 |
| Title |
Single cell RNA-seq comparing regeneration of young and aged skeletal muscles |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Abstract:
Transcription factors (TFs) play key roles in regulating differentiation and function of stem cells, including muscle satellite cells (MuSCs), a resident stem cell population responsible for postnatal regeneration of the skeletal muscle. Sox11 belongs to the Sry-related HMG-box (SOX) family of TFs that play diverse roles in stem cell behavior and tissue specification. Analysis of single-cell RNA-sequencing (scRNA-seq) datasets identify a specific enrichment of Sox11 mRNA in differentiating but not quiescent MuSCs. Consistent with the scRNA-seq data, Sox11 levels increase during differentiation of murine primary myoblasts in vitro. scRNA-seq data comparing muscle regeneration in young and old mice further demonstrate that Sox11 expression is reduced in aged MuSCs. Age-related decline of Sox11 expression is associated with reduced chromatin contacts within the topologically associated domains. Unexpectedly, Myod1Cre-driven deletion of Sox11 in embryonic myoblasts has no effects on muscle development and growth, resulting in apparently healthy muscles that regenerate normally. Pax7CreER or Rosa26CreER driven (MuSC-specific or global) deletion of Sox11 in adult mice similarly has no effects on MuSC differentiation or muscle regeneration. These results identify Sox11 as a novel myogenic differentiation marker with reduced expression in quiescent and aged MuSCs, but the specific function of Sox11 in myogenesis remain to be elucidated.
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| Overall design |
7 day post injury mononuclear cells from hindlimb skeletal muscle of young (3 months) and old (>20 months) mice were profiled by scRNA-seq and sequenced using IIIumina NovaSeq 6000
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| Contributor(s) |
Oprescu SN, Yue F, Kuang S |
| Citation(s) |
37705115 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 AR077424 |
Sox11 function in muscle stem cells |
PURDUE UNIVERSITY |
STEPHANIE NICOLE OPRESCU |
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| Submission date |
Mar 08, 2023 |
| Last update date |
Sep 21, 2023 |
| Contact name |
Shihuan Kuang |
| E-mail(s) |
[email protected]
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| Organization name |
Purdue University
|
| Street address |
901 W State St
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA942239 |
