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| Status |
Public on Apr 04, 2023 |
| Title |
Role of the CryD photoreceptor on the F. fujikuroi transcriptome |
| Organism |
Fusarium fujikuroi |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This RNA-seq data set investigates the function of the DASH cryptochrome CryD in the fungus Fusarium fujikuroi, and are an extension of the former study on the role of the WcoA photoreceptor (GEO Series GSE159533) (Pardo-Medina J, Gutiérrez G, Limón MC, Avalos J (2021) Impact of the White Collar photoreceptor WcoA on the Fusarium fujikuroi transcriptome. Front Microbiol 11:1–17. https://doi.org/10.3389/fmicb.2020.619474)). Cryptochromes are blue/UV-A light photoreceptors related to photolyases, enzymes able to use light as an energy source to repair UV-B-induced lesions. Cryptochromes typically have a N-terminal photolyase-related domain able to bind two chromophores, flavin adenine dinucleotide (FAD) and 5,10-methenyltetrahydrofolate (MTHF) or pterin, followed by a long C-terminal domain absent in photolyases, reported to play regulatory roles related to light control of growth, development, cell signaling, and circadian rhythm in different taxonomic groups. The DASH cryptochromes are a subgroup in this family that differ from cryptochromes in the ability to repair single-stranded DNA and the lack of the long carboxy extension. The Fusarium fujikuroi genome contains a single gene for a DASH cryptochrome, called CryD. In our former work, the function of the WcoA photoreceptor was investigated through the effect of the mutation of the responsible gene on the transcriptome . In this work, RNA samples of a cryD mutant were analyzed in parallel to those of the wild type and wcoA mutant described in GEO Series GSE159533, in dark and after one hour illumination. The results are summarized in the review "Fusarium photoreceptors", submitted for publication to Journal of Fungi (Basel, MDPI)
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| Overall design |
To check the role of the CryD protein, we compared the transcriptomes of the wild strain and a cryD mutant incubated in the dark or illuminated for 60 min. Data were obtained from three independent biological replicates for each condition. Data of the wild type in this study were already submitted in GEO Series GSE159533
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| Contributor(s) |
Avalos J, Medina JP, Gutiérrez G, Limón MC |
| Citation(s) |
36983487 |
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| Submission date |
Jan 20, 2023 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Javier Avalos |
| E-mail(s) |
[email protected]
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| Organization name |
University of Seville
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| Department |
Genetics
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| Street address |
Faculty of Biology, Av. Reina Mercedes s/n
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| City |
Sevilla |
| ZIP/Postal code |
41012 |
| Country |
Spain |
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| Platforms (1) |
| GPL24816 |
Illumina NextSeq 500 (Fusarium fujikuroi) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA925888 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223375_WTCRY_raw_counts.tsv.gz |
562.2 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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