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| Status |
Public on Oct 12, 2010 |
| Title |
Temporal Gene Expression Profiling during Rat Femoral Marrow Ablation-Induced Intramembranous Bone Regeneration |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Enhanced understanding of differential gene expression and biological pathways associated with distinct phases of intramembranous bone regeneration following femoral marrow ablation surgery will improve future advancements regarding osseointegration of joint replacement implants, biomaterials design, and bone tissue engineering. A rat femoral marrow ablation model was performed and genome-wide microarray data were obtained from samples at 1, 3, 5, 7, 10, 14, 28, and 56 days post-ablation, with intact bones serving as controls at Day 0. Bayesian model-based clustering produced eight distinct groups amongst 9,062 significant gene probe sets based on similar temporal expression profiles, which were further categorized into three major temporal classes of increased, variable, and decreased expression. Differential biological processes and pathways associated with each major temporal group were identified, and significantly expressed genes involved were visually represented by heat maps. It was determined that the increased expression group exclusively contains genes involved in pathways for matrix metalloproteinases (MMPs), Wnt signaling, TGF-β signaling, and inflammatory pathway. Only the variable expression group contains genes associated with glycolysis and gluconeogenesis, Notch Signaling Pathway, natural killer cell mediated cytotoxicity, and B cell receptor signaling pathway, among others. The decreased group exclusively consists of genes involved in heme biosynthesis, p53 signaling pathway, and hematopoietic cell lineage. Significant biological pathways and transcription factors expressed at each time point post-ablation were also identified.
These data present the first temporal gene expression profiling analysis of the rat genome during intramembranous bone regeneration induced by femoral marrow ablation.
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| Overall design |
In an Institutional Animal Care and Use Committee (IACUC) approved study, 27 adult male rats (Sprague Dawley, 400-425 g) were divided into nine groups: intact control (0 day), and 1, 3, 5, 7, 10, 14, 28, and 56 days post marrow ablation. Twenty-four animals received unilateral femoral ablation. At each time point, animals were killed in a carbon dioxide chamber, and whole femurs were harvested and denuded of soft tissue. For three samples per time point, both the distal and proximal ends of the femurs were cut off in order to exclude the epiphyses and growth plate regions. Diaphyseal marrow tissue and cells from the mid-shaft were flushed out using Trizol®. Extracted RNA was homoginized and was prepared for hybridization. For each of the three samples per time point, gene expression was analyzed with GeneChip® Rat Genome 230 2.0 Arrays (Affymetrix, Santa Clara, CA).
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| Contributor(s) |
Wise JK, Sena K, Vranizan K, Pollock JF, Healy KH, Hughes WF, Sumner DR, Virdi AS |
| Citation(s) |
20957030 |
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| Submission date |
Jun 14, 2010 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Amarjit S. Virdi |
| E-mail(s) |
[email protected]
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| Phone |
3129425143
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| Fax |
3129425744
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| URL |
http://www.rushu.rush.edu/servlet/Satellite?ProfileType=Detail&c=RushUnivFaculty&cid=1220880353240&pagename=Rush%2FRushUnivFaculty%2FFaculty_Staff_Profile_Detail_Page
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| Organization name |
Rush University Medical Center
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| Department |
Anatomy and Cell Biology
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| Street address |
600 South Paulina, Armour Academic Facility, Suite 507
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (27)
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| GSM555608 |
intact (control) rat femur on day 0 G1.0D.08022006-1-271 |
| GSM555609 |
intact (control) rat femur on day 0 G1.0D.18-272 |
| GSM555610 |
intact (control) rat femur on day 0 G1.0D.9-274 |
| GSM555611 |
rat femur on day 1 post-ablation G2.1D.17-266 |
| GSM555612 |
rat femur on day 1 post-ablation G2.1D.26-267 |
| GSM555613 |
rat femur on day 1 post-ablation G2.1D.8-268 |
| GSM555614 |
rat femur on day 3 post-ablation G3.3D.15-254 |
| GSM555615 |
rat femur on day 3 post-ablation G3.3D.24-255 |
| GSM555616 |
rat femur on day 3 post-ablation G3.3D.6-256 |
| GSM555617 |
rat femur on day 5 post-ablation G4.5D.16-260 |
| GSM555618 |
rat femur on day 5 post-ablation G4.5D.25-261 |
| GSM555619 |
rat femur on day 5 post-ablation G4.5D.7-262 |
| GSM555620 |
rat femur on day 7 post-ablation G5.7D.14-247 |
| GSM555621 |
rat femur on day 7 post-ablation G5.7D.23-249 |
| GSM555622 |
rat femur on day 7 post-ablation G5.7D.5-250 |
| GSM555623 |
rat femur on day 10 post-ablation G6.10D.13-242 |
| GSM555624 |
rat femur on day 10 post-ablation G6.10D.22-243 |
| GSM555625 |
rat femur on day 10 post-ablation G6.10D.4-244 |
| GSM555626 |
rat femur on day 14 post-ablation G7.14D.12-236 |
| GSM555627 |
rat femur on day 14 post-ablation G7.14D.21-237 |
| GSM555628 |
rat femur on day 14 post-ablation G7.14D.3-238 |
| GSM555629 |
rat femur on day 28 post-ablation G8.28D.11-230 |
| GSM555630 |
rat femur on day 28 post-ablation G8.28D.2-232 |
| GSM555631 |
rat femur on day 28 post-ablation G8.28D.20-231 |
| GSM555632 |
rat femur on day 56 post-ablation G9.56D.1-223 |
| GSM555633 |
rat femur on day 56 post-ablation G9.56D.10-225 |
| GSM555634 |
rat femur on day 56 post-ablation G9.56D.19-224 |
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| Relations |
| BioProject |
PRJNA127533 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22321_RAW.tar |
64.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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