|
| Status |
Public on May 14, 2023 |
| Title |
Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia [scRNA-seq PDX MLL-7] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study characterized the effect of WEE1 kinase inhibition using AZD1775 treatment on single-cell gene expression profile using the 10x Genomics protocol in the acute lymphoblastic leukemia cells
|
| |
|
| Overall design |
scRNA-seq samples were generated from NSG mice transplanted with PDX MLL-7 treated for 24 hours (2 doses) with 120 mg/kg AZD1775 (HY-10993). 4 hours after the last dose mice were sacrificed and bone marrow cells were viably frozen. Before the library preparation, freshly thaw alive hCD45pos hCD19pos mCD45neg bone marrow cells were sorted and proceeded to 10x library protocol. For combination treatments, animals were treated with 6 days of AZD1775 (MedChemExpress) at 120mg/kg alone or with Dasatinib for 3 days (MedChemExpress) at 50 mg/kg. Vehicle animals received 6 days of 5% DMSO, 40% PEG 300, 5% Tween 80 and 50% NaCl solution (all from Sigma-Aldrich). Animals were sacrificed by cervical dislocation 20 hours after the last treatment. Using fresh bone marrow cells from day 6 sequentially treated samples, the cells were FACS sorted like the shorter time point and then proceeded to the 10x library protocol.
***RAW data not provided due to patient privacy concerns***
|
| |
|
| Contributor(s) |
Lahnalampi M, Falques-Costa T, Malyukova A, Hagström A, Heinäniemi M, Sangfelt O |
| Citation(s) |
38822412 |
| |
| Submission date |
Dec 07, 2022 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Merja Heinäniemi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Eastern Finland
|
| Department |
School of Medicine, Institute of Biomedicine
|
| Lab |
Systems Genomics - Heinäniemi lab
|
| Street address |
Yliopistonranta 1E
|
| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE220114 |
Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia |
|
| Relations |
| BioProject |
PRJNA909828 |
Less...
More...