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| Status |
Public on May 31, 2024 |
| Title |
Methyl Gallate Alleviates Acute Ulcerative Colitis by Modu-lating Gut Microbiota and Inhibiting TLR4/NF-κB Pathway [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ulcerative colitis (UC) is a complex immune-mediated inflammatory disease. In recent years, the incidence of UC has increased rapidly, however, its exact etiology and mechanism are still unclear. Based on the definite anti-inflammatory and antibacterial activities of Sanguisorba officinalis L., we studied its monomer methyl gallate (MG). In this study, we employed flow cytometry and detected nitric oxide production, finding MG regulated macrophage polarization and inhibited the expression of proinflammatory cytokines in vitro. MG also exhibited anti-inflammatory activity with ameliorating body weight loss, improving colon length and histological damage in dextran sulfate sodium-induced UC mice. Meanwhile, transcription sequencing and 16S rRNA sequencing analyzed the key signaling pathways and changes in the gut microbiota of MG for UC treatment, proving MG could alleviate inflammation by regulating the TLR4/NF-κB pathway in vivo and in vitro. Additionally, MG altered the diversity and composition of the gut microbiota and changed the abundance of metabolic products. In conclusion, our results are the first to demonstrate MG has obvious therapeutic effects against acute UC, which is related to macrophage polarization, improved intestinal flora dysbiosis and inhibition of TLR4/NF-κB signaling pathway activation, and may be a promising therapeutic agent for UC treatment.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for colon tissue (normal group, model group and MG groups).
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| Contributor(s) |
Zhou P, Lai J, Zou W, Wu J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 24, 2022 |
| Last update date |
May 31, 2024 |
| Contact name |
Lai Jia |
| E-mail(s) |
[email protected]
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| Organization name |
Southwest Medical University
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| Street address |
Xianglin Road
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| City |
Luzhou |
| ZIP/Postal code |
646000 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| GSM6672672 |
Colon tissue, model_1, rep2 |
| GSM6672673 |
Colon tissue, model_2, rep2 |
| GSM6672674 |
Colon tissue, model_3, rep2 |
| GSM6672676 |
Colon tissue, MG_1, rep2 |
| GSM6672677 |
Colon tissue, MG_2, rep2 |
| GSM6672678 |
Colon tissue, MG_3, rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE216435 |
Methyl Gallate Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Inhibiting TLR4/NF-κB Pathway |
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| Relations |
| BioProject |
PRJNA893615 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216434_gene_expression_model_vs_MG.txt.gz |
4.6 Kb |
(ftp)(http) |
TXT |
| GSE216434_gene_expression_normal_vs_model.txt.gz |
35.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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