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| Status |
Public on Feb 06, 2023 |
| Title |
Distinct roles for canonical and variant histone H3 lysine 36 in polycomb silencing |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Polycomb complexes regulate cell-type specific gene expression programs through heritable silencing of target genes. Trimethylation of histone H3 lysine 27 (H3K27me3) is essential for this process. Perturbation of H3K36 is thought to interfere with H3K27me3. We show that mutants of Drosophila replication-dependent (H3.2K36R) or -independent (H3.3K36R) histone H3 genes generally maintain Polycomb silencing and reach later stages of development. In contrast, combined (H3.3K36RH3.2K36R) mutants display widespread Hox gene misexpression and fail to develop past the first larval instar. Chromatin profiling showed that the H3.2K36R mutation disrupts H3K27me3 levels broadly and significantly throughout silenced domains, whereas these regions are mostly unaffected in H3.3K36R animals. Analysis of H3.3 distributions showed that this histone is enriched at presumptive PREs (Polycomb Response Elements) located outside of silenced domains but relatively depleted from those inside. We conclude that H3.2 and H3.3 K36 residues collaborate to repress Hox genes via distinct genomic subcompartments and mechanisms.
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| Overall design |
H3K27me3 CUT&RUN of Drosophila melanogaster. Three replicates of pellet and supernatant fractions for each genotype. Genotypes: H3.3Adelta, HWT, H3.2K36R, and H3.3BK36R.
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| Contributor(s) |
Salzler HR, Vandadi V, McMichael BD, Brown JC, Boerma SA, Leatham-Jensen MP, Adams KM, Meers MP, Simon JM, Duronio RJ, McKay DJ, Matera AG |
| Citation(s) |
36857457 |
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| Submission date |
Oct 07, 2022 |
| Last update date |
Mar 22, 2023 |
| Contact name |
Gregory Matera |
| Organization name |
University of North Carolina at Chapel Hill
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| Lab |
3356
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| Street address |
Genome Science Building
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| City |
Chapel Hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (24)
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| GSM6619998 |
wing-disc, sup, HWT, rep1 |
| GSM6619999 |
wing-disc, pellet, HWT, rep1 |
| GSM6620000 |
wing-disc, sup, HWT, rep2 |
| GSM6620001 |
wing-disc, pellet, HWT, rep2 |
| GSM6620002 |
wing-disc, sup, HWT, rep3 |
| GSM6620003 |
wing-disc, pellet, HWT, rep3 |
| GSM6620004 |
wing-disc, sup, H3.2K36R, rep1 |
| GSM6620005 |
wing-disc, sup, H3.3BK36R, rep2 |
| GSM6620006 |
wing-disc, pellet, H3.2K36R, rep1 |
| GSM6620007 |
wing-disc, sup, H3.2K36R, rep2 |
| GSM6620008 |
wing-disc, pellet, H3.2K36R, rep2 |
| GSM6620009 |
wing-disc, sup, H3.2K36R, rep3 |
| GSM6620010 |
wing-disc, pellet, H3.2K36R, rep3 |
| GSM6620011 |
wing-disc, pellet, H3.3BK36R, rep1 |
| GSM6620012 |
wing-disc, sup, H3.3BK36R, rep1 |
| GSM6620013 |
wing-disc, pellet, H3.3BK36R, rep2 |
| GSM6620014 |
wing-disc, sup, H3.3BK36R, rep3 |
| GSM6620015 |
wing-disc, pellet, H3.3BK36R, rep3 |
| GSM6620016 |
wing-disc, sup, H3.3Adelta, rep1 |
| GSM6620017 |
wing-disc, pellet, H3.3Adelta, rep1 |
| GSM6620018 |
wing-disc, sup, H3.3Adelta, rep2 |
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| Relations |
| BioProject |
PRJNA888082 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE215017_RAW.tar |
587.2 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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