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Series GSE213915 Query DataSets for GSE213915
Status Public on Mar 06, 2023
Title Bombyx Vasa sequesters transposon mRNAs in nuage via phase separation requiring RNA binding and self-association [RNA-Seq]
Organism Bombyx mori
Experiment type Expression profiling by high throughput sequencing
Summary Bombyx Vasa (BmVasa) assembles non-membranous organelle, nuage or Vasa bodies, in germ cells, known as the center for Siwi-dependent transposon silencing and concomitant Ago3-piRISC biogenesis. However, details of the body assembly remain unclear. Here, we show that the N-terminal intrinsically disordered region (N-IDR) and RNA helicase domain of BmVasa are responsible for self-association and RNA binding, respectively, but N-IDR is also required for full RNA-binding activity. Both domains are essential for Vasa body assembly in vivo and droplet formation in vitro via phase separation. FAST-iCLIP reveals that BmVasa preferentially binds transposon mRNAs. Loss of Siwi function derepresses transposons but has marginal effects on BmVasa-RNA binding. This study shows that BmVasa assembles nuage by phase separation via its ability to self-associate and bind newly exported transposon mRNAs. This unique property of BmVasa allows transposon mRNAs to be sequestered and enriched in nuage, resulting in effective Siwi-dependent transposon repression and Ago3-piRISC biogenesis.
 
Overall design Total RNAs (total RNA sequencing) in BmN4 cells
 
Contributor(s) Yamazaki H, Namba Y, Siomi MC
Citation(s) 37029111
Submission date Sep 22, 2022
Last update date May 11, 2023
Contact name Yurika Namba
E-mail(s) [email protected]
Organization name The University of Tokyo
Department Department of Biological Sciences, Graduate School of Science
Lab Siomi Lab
Street address 2-11-16 Yayoi, Bunkyo-ku
City Tokyo
State/province --- Select a state ---
ZIP/Postal code 113-0032
Country Japan
 
Platforms (1)
GPL29210 Illumina NovaSeq 6000 (Bombyx mori)
Samples (4)
GSM6596459 total-RNA_Luc-KD_rep1
GSM6596460 total-RNA_Siwi-KD_rep1
GSM6596461 total-RNA_Luc-KD_rep2
This SubSeries is part of SuperSeries:
GSE213917 Bombyx Vasa sequesters transposon mRNAs in nuage via phase separation requiring RNA binding and self-association
Relations
BioProject PRJNA883173

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Supplementary file Size Download File type/resource
GSE213915_total-RNA_processed.txt.gz 445.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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