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Status |
Public on Mar 06, 2023 |
Title |
Bombyx Vasa sequesters transposon mRNAs in nuage via phase separation requiring RNA binding and self-association [RNA-Seq] |
Organism |
Bombyx mori |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Bombyx Vasa (BmVasa) assembles non-membranous organelle, nuage or Vasa bodies, in germ cells, known as the center for Siwi-dependent transposon silencing and concomitant Ago3-piRISC biogenesis. However, details of the body assembly remain unclear. Here, we show that the N-terminal intrinsically disordered region (N-IDR) and RNA helicase domain of BmVasa are responsible for self-association and RNA binding, respectively, but N-IDR is also required for full RNA-binding activity. Both domains are essential for Vasa body assembly in vivo and droplet formation in vitro via phase separation. FAST-iCLIP reveals that BmVasa preferentially binds transposon mRNAs. Loss of Siwi function derepresses transposons but has marginal effects on BmVasa-RNA binding. This study shows that BmVasa assembles nuage by phase separation via its ability to self-associate and bind newly exported transposon mRNAs. This unique property of BmVasa allows transposon mRNAs to be sequestered and enriched in nuage, resulting in effective Siwi-dependent transposon repression and Ago3-piRISC biogenesis.
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Overall design |
Total RNAs (total RNA sequencing) in BmN4 cells
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Contributor(s) |
Yamazaki H, Namba Y, Siomi MC |
Citation(s) |
37029111 |
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Submission date |
Sep 22, 2022 |
Last update date |
May 11, 2023 |
Contact name |
Yurika Namba |
E-mail(s) |
[email protected]
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Organization name |
The University of Tokyo
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Department |
Department of Biological Sciences, Graduate School of Science
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Lab |
Siomi Lab
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Street address |
2-11-16 Yayoi, Bunkyo-ku
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City |
Tokyo |
State/province |
--- Select a state --- |
ZIP/Postal code |
113-0032 |
Country |
Japan |
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Platforms (1) |
GPL29210 |
Illumina NovaSeq 6000 (Bombyx mori) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE213917 |
Bombyx Vasa sequesters transposon mRNAs in nuage via phase separation requiring RNA binding and self-association |
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Relations |
BioProject |
PRJNA883173 |